Prevalence of Stx Phages in Environments of a Pig Farm and Lysogenic Infection of the Field E. coli O157 Isolates with a Recombinant Converting Phage

Y Yan, Y Shi, D Cao, X Meng, L Xia, J Sun - Current microbiology, 2011 - Springer
The prevalence and nature of Shiga toxin (Stx)-producing Escherichia coli (STEC) and Stx
phage were investigated in 720 swine fecal samples randomly collected from a commercial …

Genetic structure and chromosomal integration site of the cryptic prophage CP-1639 encoding Shiga toxin 1

K Creuzburg, B Kohler, H Hempel… - …, 2005 - microbiologyresearch.org
The sequence of 50 625 bp of chromosomal DNA derived from Shiga-toxin (Stx)-producing
Escherichia coli (STEC) O111: H− strain 1639/77 was determined. This DNA fragment …

[图书][B] Characterization of the A subunit mutants of Stx1 and Stx2 in Saccharomyces cerevisiae

E Kyu - 2009 - search.proquest.com
Shiga toxins are a family of related toxins with two major groups, Stx1 and Stx2. The most
common sources for Shiga toxin are the bacteria Shigella dysenteriae and the …

Inducible stx2 phages are lysogenized in the enteroaggregative and other phenotypic Escherichia coli O86:HNM isolated from patients

S Iyoda, K Tamura, K Itoh, H Izumiya… - FEMS microbiology …, 2000 - academic.oup.com
We characterized two Shiga toxin-producing Escherichia coli (STEC) O86: HNM isolates
from a patient with hemolytic uremic syndrome (HUS) or bloody diarrhea. Both of them did …

A simple method for expression and purification of Shiga toxin 1 (Stx1) with biological activities by using a single‐promoter vector and native signal peptide

W Tu, T Li, Q Wang, K Cai, X Gao… - Biotechnology and …, 2016 - Wiley Online Library
The entire stx1 region from Escherichia coli O157: H7, containing two open reading frames
(stx1a and stx1b), was cloned into pET‐32a with a single promoter. This region was …

[HTML][HTML] A Single-Step Purification and Molecular Characterization of Functional Shiga Toxin 2 Variants from Pathogenic Escherichia coli

X He, B Quiñones, S McMahon, RE Mandrell - Toxins, 2012 - mdpi.com
A one-step affinity chromatography method was developed to purify Shiga toxin 2 variants
(Stx2) Stx2a, Stx2c, Stx2d and Stx2g from bacterial culture supernatants. Analysis of the …

Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157: H7 derived from the Sakai …

K Makino, K Yokoyama, Y Kubota… - Genes & genetic …, 1999 - jstage.jst.go.jp
The enterohemorrhagic Escherichia coli (EHEC) O157: H7 strain RIMD 0509952, derived
from an outbreak in Sakai city, Japan, in 1996, produces two kinds of verotoxins, VT1 and …

[HTML][HTML] Structure of Shiga toxin type 2 (Stx2) from Escherichia coli O157: H7

ME Fraser, M Fujinaga, MM Cherney… - Journal of Biological …, 2004 - ASBMB
Several serotypes of Escherichia coli produce protein toxins closely related to Shiga toxin
(Stx) from Shigella dysenteriae serotype 1. These Stx-producing E. coli cause outbreaks of …

[HTML][HTML] Comparative genomics of Shiga toxin encoding bacteriophages

DL Smith, DJ Rooks, PCM Fogg, AC Darby… - BMC genomics, 2012 - Springer
Background Stx bacteriophages are responsible for driving the dissemination of Stx toxin
genes (stx) across their bacterial host range. Lysogens carrying Stx phages can cause …

Sequence of Shiga Toxin 2 Phage 933W fromEscherichia coli O157:H7: Shiga Toxin as a Phage Late-Gene Product

G Plunkett III, DJ Rose, TJ Durfee… - Journal of …, 1999 - Am Soc Microbiol
Lysogenic bacteriophages are major vehicles for the transfer of genetic information between
bacteria, including pathogenicity and/or virulence determinants. In the enteric pathogen …