Alphavirus vectors are being developed for possible human vaccine and gene therapy
applications. We have sought to advance this field by devising DNA-based vectors and …

Sindbis virus and other alphavirus gene expression vectors have recently been used to
express and study the functions of proteins and RNA, to evaluate classical vaccine and …

High-titer alphavirus vectors have been generated for efficient gene delivery both in vitro
and in vivo. Studies on CNS infection via intranasal and peripheral injections with virulent …

Alphaviruses are RNA enveloped viruses that are proving their value as expression vectors.
They are particularly well-suited for this role as they are easily and quickly engineered and …

Several DNA-based Sindbis virus vectors were constructed to investigate the feasibility and
potential applications for initiating the virus life cycle in cells transfected directly with plasmid …

Since the recovery of infectious RNA transcripts from full-length cDNA clones, alphavirus
genome RNAs have been engineered to allow expression of heterologous RNAs and …

Alphavirus vectors based on Sindbis virus and Semliki Forest virus (SFV) were
characterized as potential gene transfer vectors. Initial studies were performed using vectors …

Alphaviruses have several features that make them attractive as gene delivery platforms,
and vectors derived principally from Sindbis virus (SIN), Semliki Forest virus (SFV), and …

The broad host cell range and high expression levels of transgenes are features that have
made alphaviruses attractive for gene expression studies and gene therapy applications …

Alphavirus expression vectors are finding novel uses in research. They are showing
increasing promise as vaccines and are being developed for diagnostic assays of other …