Since the inception of recombinant DNA technology, maximizing the solubility of a
heterologous protein has been the goal of large-scale biomanufacture1. In bacterial cell …

Background For their application in the area of diagnosis and therapy, single-domain
antibodies (sdAbs) offer multiple advantages over conventional antibodies and fragments …

Abstract Background Bacterial inclusion bodies (IBs) are key intermediates for protein
production. Their quality affects the refolding yield and further purification. Recent functional …

Expression of affinity-tagged recombinant proteins for crystallography, protein–protein
interaction, antibody generation, therapeutic applications, etc. mandates the generation of …

Background For a long time IBs were considered to be inactive deposits of accumulated
target proteins. In our previous studies, we discovered IBs containing a high percentage of …

Inclusion bodies are refractile, intracellular protein aggregates usually observed in bacteria
upon targeted gene overexpression. Since their occurrence has a major economical impact …

Extracellular production of recombinant proteins in Escherichia coli has several advantages
over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains of E …

Bacterial inclusion bodies (IBs) consist of unfolded protein aggregates and represent
inactive waste products often accumulating during heterologous overexpression of …

Background Recombinant proteins are often engineered with an N-terminal signal peptide,
which facilitates their secretion to the oxidising environment of the periplasm (gram-negative …

Background Many protocols for recombinant production of peptides and proteins include
secretion into the periplasmic space of Escherichia coli, as they may not properly fold in the …