Elongasome core proteins and class A PBP1a display zonal, processive movement at the midcell of Streptococcus pneumoniae

AJ Perez, MM Lamanna, KE Bruce… - Proceedings of the …, 2024 - National Acad Sciences
Ovoid-shaped bacteria, such as Streptococcus pneumoniae (pneumococcus), have two
spatially separated peptidoglycan (PG) synthase nanomachines that locate zonally to the …

Roles of RodZ and class A PBP1b in the assembly and regulation of the peripheral peptidoglycan elongasome in ovoid‐shaped cells of Streptococcus pneumoniae …

MM Lamanna, I Manzoor, M Joseph, ZA Ye… - Molecular …, 2022 - Wiley Online Library
RodZ of rod‐shaped bacteria functions to link MreB filaments to the Rod peptidoglycan (PG)
synthase complex that moves circumferentially perpendicular to the long cell axis, creating …

Class A PBPs have a distinct and unique role in the construction of the pneumococcal cell wall

D Straume, KW Piechowiak, S Olsen… - Proceedings of the …, 2020 - National Acad Sciences
In oval-shaped Streptococcus pneumoniae, septal and longitudinal peptidoglycan
syntheses are performed by independent functional complexes: the divisome and the …

The cell wall hydrolase Pmp23 is important for assembly and stability of the division ring in Streptococcus pneumoniae

M Jacq, C Arthaud, S Manuse, C Mercy, L Bellard… - Scientific reports, 2018 - nature.com
Bacterial division is intimately linked to synthesis and remodeling of the peptidoglycan, a
cage-like polymer that surrounds the bacterial cell, providing shape and mechanical …

Identification of pneumococcal proteins that are functionally linked to penicillin‐binding protein 2b (PBP2b)

D Straume, GA Stamsås, KH Berg… - Molecular …, 2017 - Wiley Online Library
The oval shape of pneumococci results from a combination of septal and lateral
peptidoglycan synthesis. The septal cross‐wall is synthesized by the divisome, while the …

Prevention of EloR/KhpA heterodimerization by introduction of site-specific amino acid substitutions renders the essential elongasome protein PBP2b redundant in …

AR Winther, M Kjos, GA Stamsås, LS Håvarstein… - Scientific reports, 2019 - nature.com
The RNA binding proteins EloR and KhpA are important components of the regulatory
network that controls and coordinates cell elongation and division in S. pneumoniae. Loss of …

Movement dynamics of divisome proteins and PBP2x:FtsW in cells of Streptococcus pneumoniae

AJ Perez, Y Cesbron, SL Shaw… - Proceedings of the …, 2019 - National Acad Sciences
Bacterial cell division and peptidoglycan (PG) synthesis are orchestrated by the coordinated
dynamic movement of essential protein complexes. Recent studies show that bidirectional …

Nanoscale dynamics of peptidoglycan assembly during the cell cycle of Streptococcus pneumoniae

J Trouve, A Zapun, C Arthaud, C Durmort, AM Di Guilmi… - Current Biology, 2021 - cell.com
Dynamics of cell elongation and septation are key determinants of bacterial morphogenesis.
These processes are intimately linked to peptidoglycan synthesis performed by …

EloR interacts with the lytic transglycosylase MltG at midcell in Streptococcus pneumoniae R6

AR Winther, M Kjos, ML Herigstad… - Journal of …, 2021 - Am Soc Microbiol
The ellipsoid shape of Streptococcus pneumoniae is determined by the synchronized
actions of the elongasome and the divisome, which have the task of creating a protective …

Organization of peptidoglycan synthesis in nodes and separate rings at different stages of cell division of Streptococcus pneumoniae

AJ Perez, MJ Boersma, KE Bruce… - Molecular …, 2021 - Wiley Online Library
Bacterial peptidoglycan (PG) synthesis requires strict spatiotemporal organization to
reproduce specific cell shapes. In ovoid‐shaped Streptococcus pneumoniae (Spn), septal …