For interpretation of quantitative gene expression measurements in clinical tumor samples, a
normalizer is necessary to correct expression data for differences in cellular input, RNA …

Accurate normalization is an absolute prerequisite for correct measurement of gene
expression. For quantitative real-time reverse transcription-PCR (RT-PCR), the most …

Using quantitative reverse transcription–polymerase chain reaction (RT-PCR), reference
genes are utilized as endogenous controls for relative quantification of target genes in gene …

Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) represents a sensitive
and efficient technique to determine expression levels of target genes in multiple samples …

Background: Differences in gene expression are frequently encountered in malignant
tissues, and have been intensively studied as they can reflect different experimental or …

In quantitative reverse transcription–polymerase chain reaction (qRT–PCR), normalization
using reference genes is a common useful approach, but the validation of suitable reference …

Real-time reverse transcription PCR (RT-PCR) is a sensitive and accurate method to
monitor gene expression and is often used to profile the expression of putative tumor …

Background Considering the broad variation in the expression of housekeeping genes
among tissues and experimental situations, studies using quantitative RT-PCR require strict …

To standardize the amount of biological material between samples (eg, number of cells or
amount of tissue) for quantitative real-time reverse transcriptase PCR (qRT-PCR), the cycle …

Cancer is a genetic disease where genetic variations cause abnormally functioning genes
that appear to alter expression. Proteins, the final products of gene expression, determine …