Docking sites inside Cas9 for adenine base editing diversification and RNA off-target elimination
S Li, B Yuan, J Cao, J Chen, J Chen, J Qiu… - Nature …, 2020 - nature.com
Base editing tools with diversified editing scopes and minimized RNA off-target activities are
required for broad applications. Nevertheless, current Streptococcus pyogenes Cas9 …
required for broad applications. Nevertheless, current Streptococcus pyogenes Cas9 …
Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Applications of adenine base editors (ABEs) have been constrained by the limited
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …
Engineering domain-inlaid SaCas9 adenine base editors with reduced RNA off-targets and increased on-target DNA editing
MT Nguyen Tran, MKN Mohd Khalid, Q Wang… - Nature …, 2020 - nature.com
Precision genome engineering has dramatically advanced with the development of
CRISPR/Cas base editing systems that include cytosine base editors and adenine base …
CRISPR/Cas base editing systems that include cytosine base editors and adenine base …
nCas9 Engineering for Improved Target Interaction Presents an Effective Strategy to Enhance Base Editing
G Zhang, Z Song, S Huang, Y Wang, J Sun… - Advanced …, 2024 - Wiley Online Library
Base editors (BEs) are a recent generation of genome editing tools that couple a cytidine or
adenosine deaminase activity to a catalytically impaired Cas9 moiety (nCas9) to enable …
adenosine deaminase activity to a catalytically impaired Cas9 moiety (nCas9) to enable …
Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors
Base editing requires that the target sequence satisfy the protospacer adjacent motif
requirement of the Cas9 domain and that the target nucleotide be located within the editing …
requirement of the Cas9 domain and that the target nucleotide be located within the editing …
TadA orthologs enable both cytosine and adenine editing of base editors
S Zhang, B Yuan, J Cao, L Song, J Chen, J Qiu… - Nature …, 2023 - nature.com
Cytidine and adenosine deaminases are required for cytosine and adenine editing of base
editors respectively, and no single deaminase could enable concurrent and comparable …
editors respectively, and no single deaminase could enable concurrent and comparable …
Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors
KN Berríos, A Barka, J Gill, JC Serrano… - Nucleic Acids …, 2024 - academic.oup.com
The partnership of DNA deaminase enzymes with CRISPR-Cas nucleases is now a well-
established method to enable targeted genomic base editing. However, an understanding of …
established method to enable targeted genomic base editing. However, an understanding of …
Expanding the genome-targeting scope and the site selectivity of high-precision base editors
Base editors (BEs) are RNA-guided CRISPR-Cas-derived genome editing tools that induce
single-nucleotide changes. The limitations of current BEs lie in their low precision …
single-nucleotide changes. The limitations of current BEs lie in their low precision …
BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering.
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …
A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9
Y Qian, D Wang, W Niu, D Zhao, J Li, Z Liu, X Gao… - BMC biology, 2023 - Springer
Abstract Background Adenine base editors (ABEs) are promising therapeutic gene editing
tools that can efficiently convert targeted A• T to G• C base pairs in the genome. However …
tools that can efficiently convert targeted A• T to G• C base pairs in the genome. However …