A variety of techniques for strain engineering in Saccharomyces cerevisiae have recently
been developed. However, especially when multiple genetic manipulations are required …

CRISPR-Cas has become a powerful technique for genetic engineering of yeast. Here, we
present an improved version by using only one single plasmid expressing Cas9 and one or …

Recent advances in CRISPR/Cas9 based genome editing have considerably advanced
genetic engineering of industrial yeast strains. In this study, we report the construction and …

One-step multiple gene disruption in the model organism Saccharomyces cerevisiae is a
highly useful tool for both basic and applied research, but it remains a challenge. Here, we …

Saccharomyces cerevisiae has been for a long time a common model for fundamental
biological studies and a popular biotechnological engineering platform to produce …

The methods based on the clustered regularly interspaced short palindromic repeats
(CRISPR)/CRISPR-associated (Cas) system have quickly gained popularity for genome …

CRISPR-Cas genome engineering in yeast has relied on preparation of complex expression
plasmids for multiplexed gene knockouts and point mutations. Here we show that co …

Global demand has driven the use of industrial strains of the yeast Saccharomyces
cerevisiae for large-scale production of biofuels and renewable chemicals. However, the …

Genome modification in budding yeast has been extremely successful largely due to its
highly efficient homology-directed DNA repair machinery. Several methods for modifying the …

While CRISPR-Cas9-mediated genome editing has transformed yeast research, current
plasmids and cassettes for Cas9 and guide-RNA expression are species specific. CRISPR …