Analysis of cell-based RNAi screens
RNA interference (RNAi) screening is a powerful technology for functional characterization
of biological pathways. Interpretation of RNAi screens requires computational and statistical …
of biological pathways. Interpretation of RNAi screens requires computational and statistical …
Design and evaluation of genome-wide libraries for RNA interference screens
RNA interference (RNAi) screens have enabled the systematic analysis of many biological
processes in cultured cells and whole organisms. The success of such screens and the …
processes in cultured cells and whole organisms. The success of such screens and the …
Genomic screening with RNAi: results and challenges
S Mohr, C Bakal, N Perrimon - Annual review of biochemistry, 2010 - annualreviews.org
RNA interference (RNAi) is an effective tool for genome-scale, high-throughput analysis of
gene function. In the past five years, a number of genome-scale RNAi high-throughput …
gene function. In the past five years, a number of genome-scale RNAi high-throughput …
GenomeRNAi: a database for cell-based and in vivo RNAi phenotypes, 2013 update
EE Schmidt, O Pelz, S Buhlmann, G Kerr… - Nucleic acids …, 2013 - academic.oup.com
RNA interference (RNAi) represents a powerful method to systematically study loss-of-
function phenotypes on a large scale with a wide variety of biological assays, constituting a …
function phenotypes on a large scale with a wide variety of biological assays, constituting a …
Applications of high-throughput RNA interference screens to problems in cell and developmental biology
N Perrimon, B Mathey-Prevot - Genetics, 2007 - academic.oup.com
RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology
for identifying gene functions systematically and in an unbiased manner. Here, we describe …
for identifying gene functions systematically and in an unbiased manner. Here, we describe …
High-throughput RNAi screening in cultured cells: a user's guide
CJ Echeverri, N Perrimon - Nature Reviews Genetics, 2006 - nature.com
RNA interference has re-energized the field of functional genomics by enabling genome-
scale loss-of-function screens in cultured cells. Looking back on the lessons that have been …
scale loss-of-function screens in cultured cells. Looking back on the lessons that have been …
The art and design of genetic screens: RNA interference
M Boutros, J Ahringer - Nature Reviews Genetics, 2008 - nature.com
The remarkable gene knockdown technique of RNAi has opened exciting new avenues for
genetic screens in model organisms and human cells. Here we describe the current state of …
genetic screens in model organisms and human cells. Here we describe the current state of …
Statistical methods for analysis of high-throughput RNA interference screens
A Birmingham, LM Selfors, T Forster, D Wrobel… - Nature …, 2009 - nature.com
RNA interference (RNAi) has become a powerful technique for reverse genetics and drug
discovery, and in both of these areas large-scale high-throughput RNAi screens are …
discovery, and in both of these areas large-scale high-throughput RNAi screens are …
RNAi screening: new approaches, understandings, and organisms
SE Mohr, N Perrimon - Wiley Interdisciplinary Reviews: RNA, 2012 - Wiley Online Library
RNA interference (RNAi) leads to sequence‐specific knockdown of gene function. The
approach can be used in large‐scale screens to interrogate function in various model …
approach can be used in large‐scale screens to interrogate function in various model …
Minimizing the risk of reporting false positives in large-scale RNAi screens
Large-scale RNA interference (RNAi)-based analyses, very much as other'omic'approaches,
have inherent rates of false positives and negatives. The variability in the standards of care …
have inherent rates of false positives and negatives. The variability in the standards of care …