Analysis of cell-based RNAi screens

M Boutros, LP Brás, W Huber - Genome biology, 2006 - Springer
RNA interference (RNAi) screening is a powerful technology for functional characterization
of biological pathways. Interpretation of RNAi screens requires computational and statistical …

Design and evaluation of genome-wide libraries for RNA interference screens

T Horn, T Sandmann, M Boutros - Genome biology, 2010 - Springer
RNA interference (RNAi) screens have enabled the systematic analysis of many biological
processes in cultured cells and whole organisms. The success of such screens and the …

Genomic screening with RNAi: results and challenges

S Mohr, C Bakal, N Perrimon - Annual review of biochemistry, 2010 - annualreviews.org
RNA interference (RNAi) is an effective tool for genome-scale, high-throughput analysis of
gene function. In the past five years, a number of genome-scale RNAi high-throughput …

GenomeRNAi: a database for cell-based and in vivo RNAi phenotypes, 2013 update

EE Schmidt, O Pelz, S Buhlmann, G Kerr… - Nucleic acids …, 2013 - academic.oup.com
RNA interference (RNAi) represents a powerful method to systematically study loss-of-
function phenotypes on a large scale with a wide variety of biological assays, constituting a …

Applications of high-throughput RNA interference screens to problems in cell and developmental biology

N Perrimon, B Mathey-Prevot - Genetics, 2007 - academic.oup.com
RNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology
for identifying gene functions systematically and in an unbiased manner. Here, we describe …

High-throughput RNAi screening in cultured cells: a user's guide

CJ Echeverri, N Perrimon - Nature Reviews Genetics, 2006 - nature.com
RNA interference has re-energized the field of functional genomics by enabling genome-
scale loss-of-function screens in cultured cells. Looking back on the lessons that have been …

The art and design of genetic screens: RNA interference

M Boutros, J Ahringer - Nature Reviews Genetics, 2008 - nature.com
The remarkable gene knockdown technique of RNAi has opened exciting new avenues for
genetic screens in model organisms and human cells. Here we describe the current state of …

Statistical methods for analysis of high-throughput RNA interference screens

A Birmingham, LM Selfors, T Forster, D Wrobel… - Nature …, 2009 - nature.com
RNA interference (RNAi) has become a powerful technique for reverse genetics and drug
discovery, and in both of these areas large-scale high-throughput RNAi screens are …

RNAi screening: new approaches, understandings, and organisms

SE Mohr, N Perrimon - Wiley Interdisciplinary Reviews: RNA, 2012 - Wiley Online Library
RNA interference (RNAi) leads to sequence‐specific knockdown of gene function. The
approach can be used in large‐scale screens to interrogate function in various model …

Minimizing the risk of reporting false positives in large-scale RNAi screens

CJ Echeverri, PA Beachy, B Baum, M Boutros… - Nature …, 2006 - nature.com
Large-scale RNA interference (RNAi)-based analyses, very much as other'omic'approaches,
have inherent rates of false positives and negatives. The variability in the standards of care …