Cryobiotechnology of apple (Malus spp.): development, progress and future prospects

MR Wang, L Chen, JA Teixeira da Silva, GM Volk… - Plant Cell Reports, 2018 - Springer
Key message Cryopreservation provides valuable genes for further breeding of elite
cultivars, and cryotherapy improves the production of virus-free plants in Malus spp., thus …

[PDF][PDF] Cryopreservation of apple genetic resources

C Stushnoff - Canadian Journal of Plant Science, 1987 - cdnsciencepub.com
CRYOPRESERVATION OF APPLE GENETIC RESOURCES Page 1 CRYOPRESERVATION OF
APPLE GENETIC RESOURCES C. STUSHNOFF Department of Horticulture Science, lJniversity …

Cold acclimation improves regrowth of cryopreserved apple shoot tips

SV Kushnarenko, NV Romadanova, BM Reed - CryoLetters, 2009 - ingentaconnect.com
Cryopreservation is important for safeguarding the genetic resources of apple germplasm in
Kazakhstan, the center of origin for apples. In this study, conducted with five apple …

Eradication of latent viruses from apple cultivar 'Monalisa'shoot tips using droplet-vitrification cryotherapy

JC Bettoni, JA Souza, GM Volk, M Dalla Costa… - Scientia …, 2019 - Elsevier
In apple, virally transmitted diseases cause economic losses as a result of their direct effect
on production and yield and by causing an increased susceptibility to other phytopathogenic …

Cryopreservation of Malus cultivars: comparison of two droplet protocols

A Halmagyi, C Deliu, V Isac - Scientia horticulturae, 2010 - Elsevier
Two droplet procedures, droplet-vitrification (PVS2) and droplet (DMSO) were applied for
cryopreservation of in vitro cultured apple (Malus domestica Borkh., cvs. Florina, Idared …

Cryotherapy as a method for reducing the virus infection of apples (Malus sp.)

NV Romadanova, SA Mishustina, DA Gritsenko… - …, 2016 - ingentaconnect.com
BACKGROUND: There is an urgent need in Kazakhstan for virus-free nursery stock to
reinvigorate the industry and preserve historic cultivars. An in vitro collection of apples could …

[HTML][HTML] Cryotherapy by encapsulation-dehydration is effective for in vitro eradication of latent viruses from 'Marubakaido'apple rootstock

JC Bettoni, M Dalla Costa, JA Souza, GM Volk… - Journal of …, 2018 - Elsevier
Apple stem pitting virus (ASPV), Apple chlorotic leaf spot virus (ACLSV) and Apple stem
grooving virus (ASGV) are several major viral pathogens of apple trees, responsible for …

Cryopreservation of fruit germplasm

M Höfer, MV Hanke - In Vitro Cellular & Developmental Biology-Plant, 2017 - Springer
Most temperate fruit species are genetically heterozygous and vegetatively propagated.
Active collections of fruit genetic resources in Germany are generally maintained in the field …

Duration of sucrose preculture is critical for shoot regrowth of in vitro-grown apple shoot-tips cryopreserved by encapsulation-dehydration

CH Feng, ZH Cui, BQ Li, L Chen, YL Ma… - Plant Cell, Tissue and …, 2013 - Springer
A simple and efficient cryopreservation protocol using encapsulation-dehydration was
established for in vitro-grown shoot-tips of apple 'Gala'(Malus× domestica Borkh.). Shoot …

Cryopreservation of winter-dormant apple buds: establishment of a duplicate collection of Malus germplasm

M Höfer - Plant Cell, Tissue and Organ Culture (PCTOC), 2015 - Springer
The clonal wild-species Malus collection at the Julius Kühn-Institute, Institute for Breeding
Research on Fruit Crops, Germany, comprises 507 accessions of 46 species and represents …