Nested reverse transcriptase‐polymerase chain reaction for the detection of group A rotaviruses

M Elschner, J Prudlo, H Hotzel, P Otto… - Journal of Veterinary …, 2002 - Wiley Online Library
Rotaviruses are important pathogens associated with diarrhoeal diseases in almost all
species of mammals. In the present study, a nested reverse transcriptase‐polymerase chain …

PCR detection of group A bovine rotaviruses in feces

J Chinsangaram, GY Akita… - Journal of Veterinary …, 1993 - journals.sagepub.com
A polymerase chain reaction (PCR) protocol has been developed for identification of bovine
group A rotavirus infection in feces. Primers (20mers) complementary to 3′ ends of double …

Verification of sensitivity and specificity of group A rotavirus detection in piglets faeces with monoclonal blocking ELISA methods

L Rodák, B Šmíd, Z Nevoránková… - Journal of Veterinary …, 2004 - Wiley Online Library
Monoclonal antibodies to group A rotavirus Vp6 protein were prepared and used for
verification of three blocking enzyme‐linked immunosorbent assay (ELISA) modifications to …

A novel multiplex RT-PCR for identification of VP6 subgroups of human and porcine rotaviruses

A Thongprachum, N Chaimongkol, P Khamrin… - Journal of virological …, 2010 - Elsevier
VP6 protein antigens allow classification of rotaviruses into at least four subgroups,
depending on the presence or absence of SG-specific epitopes: SG I, SG II, SG (I+ II), and …

Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2

RB Bellinzoni, NM Mattion, DO Matson… - Journal of Clinical …, 1990 - Am Soc Microbiol
Fecal samples from rotavirus-infected piglets were characterized by a serotyping enzyme-
linked immunosorbent assay (ELISA) by using monoclonal antibodies (MAbs) specific to …

Detection of group B and C rotaviruses by polymerase chain reaction

V Gouvea, JR Allen, RI Glass, ZY Fang… - Journal of Clinical …, 1991 - Am Soc Microbiol
We adapted the polymerase chain reaction (PCR) to detect the noncultivatable group B and
C rotaviruses and introduced a simple and convenient technique to purify viral RNA from …

Identification of human and bovine rotavirus serotypes by polymerase chain reaction

K Taniguchi, F Wakasugi, Y Pongsuwanna… - Epidemiology & …, 1992 - cambridge.org
The use of the polymerase chain reaction (PCR) for identifying serotypes of human and
bovine rotaviruses was examined. In the identification of 115 human rotavirus samples in …

Characterization of group C rotaviruses associated with diarrhea outbreaks in feeder pigs

Y Kim, KO Chang, B Straw, LJ Saif - Journal of clinical …, 1999 - Am Soc Microbiol
Feces and serum specimens were collected from three farms in Michigan on which∼ 50-lb
(8-to 9-week-old) pigs experienced diarrhea just after placement into all-in-all-out finishing …

Amplification of all 11 RNA segments of group A rotaviruses based on reverse transcription polymerase chain reaction

Y Fujii, T Shimoike, H Takagi… - Microbiology and …, 2012 - Wiley Online Library
Group A rotaviruses (RVA) are a major cause of acute infantile gastroenteritis. The viral
genome comprises 11 double‐stranded RNA segments and the respective gene segments …

Detection of bovine group B rotaviruses in feces by polymerase chain reaction

J Chinsangaram, GY Akita… - Journal of veterinary …, 1994 - journals.sagepub.com
A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus
(IDIR) were employed to amplify genome segment 9 of a group B bovine rotavirus in a …