真核表达CatSper1 用于筛选有效siRNA 的体外实验研究
梅小琴, 李红钢, 熊承良 - 生殖医学杂志, 2011 - cqvip.com
目的构建pEGFP-N1-CatSper1 重组质粒, 在真核细胞中表达CatSper1, 利用化学合成siRNA
抑制小鼠CatSper1 基因的表达, 并筛选抑制效果最佳的siRNA 序列. 方法利用重组PCR …
抑制小鼠CatSper1 基因的表达, 并筛选抑制效果最佳的siRNA 序列. 方法利用重组PCR …
精子CatSper2 的表达水平与不同精液参数及妊娠结局关系的初步研究
李伟伟, 史鸿志, 殷秀荣, 闫娅妮, 马波 - 生殖医学杂志, 2018 - cqvip.com
目的探讨CatSper2 基因的表达水平与助孕妊娠结局的关系. 方法回顾性分析2016 年4
月至2018 年2 月在我院生殖中心进行常规IVF 的112 例夫妇的临床资料, 并根据不同精液参数 …
月至2018 年2 月在我院生殖中心进行常规IVF 的112 例夫妇的临床资料, 并根据不同精液参数 …
[HTML][HTML] Catsper1 promoter is bidirectional and regulates the expression of a novel lncRNA
SE Jiménez-Badillo, N Oviedo… - Scientific reports, 2017 - nature.com
The Catsper1 gene, whose expression is restricted to male germ cells, has great importance
in reproductive biology because of its function in sperm motility and fertilization. We …
in reproductive biology because of its function in sperm motility and fertilization. We …
Optimization of real time RT-PCR system for the quantitative estimation of CatSper1 mRNA levels in human and mouse mature spermatozoa
HG Li, AH Liao, XB Kong, L Hu… - Zhonghua nan ke xue …, 2007 - europepmc.org
Objective To establish and optimize a real time RT-PCR system for determining the
transcript levels of CatSper1 in human and mouse mature spermatozoa containing …
transcript levels of CatSper1 in human and mouse mature spermatozoa containing …
用SYBR Green I 实时逆转录-聚合酶链反应定量检测人, 小鼠精子中CatSper1 mRNA
李红钢, 廖爱华, 孔祥斌, 胡廉, 熊承良 - 中华男科学杂志, 2007 - cqvip.com
目的: 建立并优化SYBR GreenI 实时RT-PCR 体系, 定量检测人, 小鼠成熟精子中的CatSper1
mRNA. 方法: 用TRIzol 分别提取人, 小鼠成熟精子中的总RNA, 逆转录后用SYBR GreenI …
mRNA. 方法: 用TRIzol 分别提取人, 小鼠成熟精子中的总RNA, 逆转录后用SYBR GreenI …
Yijingfang increases the expression of CatSper1 in mice
SB Luo, JM Jia, HX Hu, WG Ma, YZ Jiao… - Zhonghua nan ke xue …, 2010 - europepmc.org
Objective To observe the effects of Yijingfang on CatSper1 in the mouse model of
cyclophosphamide-induced oligoasthenospermia. Methods Forty Kunming male mice were …
cyclophosphamide-induced oligoasthenospermia. Methods Forty Kunming male mice were …
[HTML][HTML] Regulation of fertilization in male rats by CatSper2 knockdown
Z Zhang, GL Wang, HX Li, L Li, QW Cui… - Asian journal of …, 2012 - ncbi.nlm.nih.gov
Interest in ion channels as drug targets for contraception has grown with the realization that
certain ion channel subunits are located exclusively in sperm. Selective knockdown of ion …
certain ion channel subunits are located exclusively in sperm. Selective knockdown of ion …