Two essential transfer genes of the conjugative plasmid RP4 were altered by site-directed
mutagenesis: traG of the primase operon and traI of the relaxase operon. To evaluate effects …

Conjugative DNA transfer is a highly conserved process for the direct transfer of DNA from a
donor to a recipient. The conjugative initiator proteins are key players in the DNA processing …

Bacterial conjugation is a promiscuous DNA transport mechanism. Conjugative plasmids
transfer themselves between most bacteria, thus being one of the main causal agents of the …

F plasmid‐mediated bacterial conjugation requires interactions between a relaxosome
component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the …

Conjugative relaxases are the proteins that initiate bacterial conjugation by a site-specific
cleavage of the transferred DNA strand. In vitro, they show strand-transferase activity on …

To characterize protein-DNA interactions involved in the initiation of conjugative transfer
replication we isolated and dissected the transfer origins (oriT) of the promiscuous IncP …

TrwC, the relaxase of plasmid R388, catalyzes a series of concerted DNA cleavage and
strand transfer reactions on a specific site (nic) of its origin of transfer (oriT). nic contains the …

We show that relaxase TrwC promotes recombination between two directly repeated oriTs
while related relaxases TraI of F and pKM101 do not. Efficient recombination required also …

Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the
relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system …

The plasmids R1162 and pSC101 have origins of conjugative transfer (oriT s) and
corresponding relaxases that are closely related. The oriT s are made up of a highly …