Molecular cloning and expression of a second chondroitin N-acetylgalactosaminyltransferase involved in the initiation and elongation of chondroitin/dermatan sulfate
T Uyama, H Kitagawa, J Tanaka, J Tamura… - Journal of Biological …, 2003 - ASBMB
We identified a novel human chondroitinN-acetylgalactosaminyltransferase, designated
chondroitin GalNAcT-2 after a BLAST analysis of the GenBank TM data base using the …
chondroitin GalNAcT-2 after a BLAST analysis of the GenBank TM data base using the …
Molecular Cloning and Expression of Human ChondroitinN-Acetylgalactosaminyltransferase: THE KEY ENZYME FOR CHAIN INITIATION AND ELONGATION OF …
T Uyama, H Kitagawa, J Tamura, K Sugahara - Journal of Biological …, 2002 - ASBMB
Based on sequence homology with the recently cloned human chondroitin synthase, we
identified a novel β1, 4-N-acetylgalactosaminyltransferase, which consisted of 532 amino …
identified a novel β1, 4-N-acetylgalactosaminyltransferase, which consisted of 532 amino …
Enzymatic synthesis of chondroitin with a novel chondroitin sulfate N-acetylgalactosaminyltransferase that transfers N-acetylgalactosamine to glucuronic acid in …
M Gotoh, T Sato, T Akashima, H Iwasaki… - Journal of Biological …, 2002 - ASBMB
We found a novel glycosyltransferase gene having a hypothetical β1, 4-
galactosyltransferase motif (GenBank TM accession number AB081516) by a BLAST search …
galactosyltransferase motif (GenBank TM accession number AB081516) by a BLAST search …
Differential Roles of TwoN-Acetylgalactosaminyltransferases, CSGalNAcT-1, and a Novel Enzyme, CSGalNAcT-2: Initiation and Elongation in Synthesis of Chondroitin …
T Sato, M Gotoh, K Kiyohara, T Akashima… - Journal of Biological …, 2003 - ASBMB
By a tblastn search with β1, 4-galactosyltransferases as query sequences, we found an
expressed sequence tag that showed similarity in β1, 4-glycosyltransferase motifs. The full …
expressed sequence tag that showed similarity in β1, 4-glycosyltransferase motifs. The full …
Molecular cloning of a chondroitin polymerizing factor that cooperates with chondroitin synthase for chondroitin polymerization
H Kitagawa, T Izumikawa, T Uyama… - Journal of Biological …, 2003 - ASBMB
We recently cloned human chondroitin synthase (ChSy) exhibiting the glucuronyltransferase-
II (GlcATII) and N-acetylgalactosaminyltransferase-II (GalNAcTII) activities responsible for …
II (GlcATII) and N-acetylgalactosaminyltransferase-II (GalNAcTII) activities responsible for …
Chondroitin sulfate synthase-2: molecular cloning and characterization of a novel human glycosyltransferase homologous to chondroitin sulfate glucuronyltransferase …
T Yada, M Gotoh, T Sato, M Shionyu, M Go… - Journal of Biological …, 2003 - ASBMB
Chondroitin sulfate is found in a variety of tissues as proteoglycans and consists of repeating
disaccharide units of N-acetylgalactosamine and glucuronic acid residues with sulfate …
disaccharide units of N-acetylgalactosamine and glucuronic acid residues with sulfate …
Chondroitin sulfate N-acetylgalactosaminyltransferase-1 (CSGalNAcT-1) involved in chondroitin sulfate initiation: Impact of sulfation on activity and specificity
S Gulberti, JC Jacquinet, M Chabel… - …, 2012 - academic.oup.com
Glycosaminoglycan (GAG) assembly initiates through the formation of a linkage
tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan …
tetrasaccharide region serving as a primer for both chondroitin sulfate (CS) and heparan …
Identification of chondroitin sulfate glucuronyltransferase as chondroitin synthase-3 involved in chondroitin polymerization: chondroitin polymerization is achieved by …
T Izumikawa, T Koike, S Shiozawa, K Sugahara… - Journal of Biological …, 2008 - ASBMB
Recently, we demonstrated that chondroitin polymerization is achieved by any two
combinations of human chondroitin synthase-1 (ChSy-1), ChSy-2 (chondroitin sulfate …
combinations of human chondroitin synthase-1 (ChSy-1), ChSy-2 (chondroitin sulfate …
Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin …
T Izumikawa, T Uyama, Y Okuura… - Biochemical …, 2007 - portlandpress.com
Previously, we have demonstrated that co-expression of ChSy-1 (chondroitin synthase-1),
with ChPF (chondroitin-polymerizing factor) resulted in a marked augmentation of …
with ChPF (chondroitin-polymerizing factor) resulted in a marked augmentation of …
Regulation of chondroitin sulfate biosynthesis by specific sulfation: acceptor specificity of serum β-GalNAc transferase revealed by structurally defined …
H Kitagawa, K Tsutsumi, M Ujikawa, F Goto… - …, 1997 - academic.oup.com
The relationship between sulfation and polymerization in chondroitin sulfate (CS)
biosynthesis has been poorly understood. In this study, we investigated the specificity of …
biosynthesis has been poorly understood. In this study, we investigated the specificity of …