A major challenge in attaching fluorophores or other handles to proteins is the availability of
a site-specific labeling strategy that provides stoichiometric modification without …

For a long time, the total synthesis of proteins was considered as a “mission impossible”
because of the tedious and complex synthetic steps and demanding purification processes …

NOTE: In the version of this Review initially published, an author (B. Martin Hallberg) was left
off of the author list. This information has been added to the HTML and PDF versions of the …

The isolation of proteins in high yield and purity is a major bottleneck for the analysis of their
three-dimensional structure, function and interactome. Here, we present a streamlined …

In selecting a method to produce a recombinant protein, a researcher is faced with a
bewildering array of choices as to where to start. To facilitate decision-making, we describe …

Screening strategies based on functional genomics require the isolation of gene products of
several hundred cDNA clones in a fast and versatile manner. Conventional purification …

Over the past decade, a new protein purification technique has emerged as a result of
recombinant DNA technology. DNA, encoding additional polypeptide or protein tags, is …

A protein-cleaving catalyst specific for a disease-related protein can be used as a catalytic
drug. As the first protein-cleaving catalyst selective for a protein substrate, a catalyst for …

Facilitating the process of chemical protein synthesis is an important goal in order to enable
the efficient preparation of large and novel protein analogues. Native chemical ligation …

The maintenance of machinery requires its operational understanding and a toolbox for
repair. The methods for the precision engineering of native proteins meet a similar …