Formation of relaxosomes is the first step in the initiation of transfer DNA replication during
bacterial conjugation. This nucleoprotein complex contains all components capable of …

During initiation of conjugative transfer of DNA containing the transfer origin (oriT) of the
promiscuous plasmid RP4, the proteins TraI, TraJ, and TraH interact and assemble a …

Transfer of plasmid RP4 during bacterial conjugation requires the plasmid-encoded TraJ
protein, which binds to the transfer origin (Fürste, JP, Pansegrau, W., Ziegelin, G., Kröger, M …

Two essential transfer genes of the conjugative plasmid RP4 were altered by site-directed
mutagenesis: traG of the primase operon and traI of the relaxase operon. To evaluate effects …

The TraI protein of plasmid RP4 (IncP alpha) catalyzes a site-and strand-specific cleaving-
joining reaction on form I or single-stranded DNA. Thus, TraI is one of the key components …

Conjugative DNA transfer of the self-transmissible broad-host-range plasmid RP4 is initiated
by strand-and site-specific cleavage at the nick site (nic) of the transfer origin (oriT) …

Conjugative transfer of the self-transmissible IncP plasmid RP4 requires the product of the
RP4 traK gene. By using the phage T7 expression system, the traK gene product was …

To characterize protein-DNA interactions involved in the initiation of conjugative transfer
replication we isolated and dissected the transfer origins (oriT) of the promiscuous IncP …

The relaxase (TraI) of plasmid RP4 (IncPα) plays a key role in initiation and termination of
transfer DNA replication during conjugative transmission of the plasmid. TraI functions as a …

Conjugative DNA transfer is a highly conserved process for the direct transfer of DNA from a
donor to a recipient. The conjugative initiator proteins are key players in the DNA processing …