Transcription from Fusion Promoters Generated during Transposition of Transposon Tn4652 Is Positively Affected by Integration Host Factor in Pseudomonas putida

R Teras, R Hõrak, M Kivisaar - Journal of bacteriology, 2000 - Am Soc Microbiol
We have previously shown that both ends of the Tn 3 family transposon Tn 4652 contain
integration host factor (IHF) binding sites and that IHF positively regulates expression of the …

Expression of the Transposase Gene tnpAof Tn4652 Is Positively Affected by Integration Host Factor

R Hõrak, M Kivisaar - Journal of bacteriology, 1998 - Am Soc Microbiol
Tn 4652 is a derivative of the toluene degradation transposon Tn 4651 that belongs to the
Tn 3 family of transposons (M. Tsuda and T. Iino, Mol. Gen. Genet. 210: 270–276, 1987). We …

Regulation of the Transposase of Tn4652by the Transposon-Encoded Protein TnpC

R Hõrak, M Kivisaar - Journal of bacteriology, 1999 - Am Soc Microbiol
Transposition is a DNA reorganization reaction potentially deleterious for the host. The
frequency of transposition is limited by the amount of transposase. Therefore, strict …

IHF is the limiting host factor in transposition of Pseudomonas putida transposon Tn4652 in stationary phase

H Ilves, R Hõrak, R Teras, M Kivisaar - Molecular microbiology, 2004 - Wiley Online Library
Transpositional activity of mobile elements is not constant. Conditional regulation of host
factors involved in transposition may severely change the activity of mobile elements. We …

Fis negatively affects binding of Tn4652 transposase by out-competing IHF from the left end of Tn4652

R Teras, J Jakovleva, M Kivisaar - Microbiology, 2009 - microbiologyresearch.org
Transposition activity in bacteria is generally maintained at a low level. The activity of mobile
DNA elements can be controlled by bacterially encoded global regulators. Regulation of …

Target Site Selection of Pseudomonas putida Transposon Tn4652

PA Kivistik, M Kivisaar, R Horak - Journal of bacteriology, 2007 - Am Soc Microbiol
We analyzed the target preferences of a Tn 3 family transposon Tn 4652. Alignment of 93
different insertion sites revealed a consensus sequence which resembles that of Tn 3 …

Conjugative transposition of Tn916: the transposon int gene is required only in the donor

F Bringel, GL Van Alstine, JR Scott - Journal of bacteriology, 1992 - Am Soc Microbiol
Conjugative transposition of transposon Tn916 has been shown to proceed by excision of
the transposon in the donor strain and insertion of this element in the recipient. This process …

Preferential transposition of an IS630-associated composite transposon to TA in the 5'-CTAG-3'sequence

T Tenzen, E Ohtsubo - Journal of bacteriology, 1991 - Am Soc Microbiol
A composite transposon, Tn4731, associated with IS630 has been shown to transpose
preferentially to 5'-TA-3'sequences that are located at two sites in a rho-dependent …

Transposition of Tn1000: in vivo properties

MM Tsai, RY Wong, AT Hoang… - Journal of …, 1987 - Am Soc Microbiol
Transposition mediated by the Tn1000 transposase was investigated by using transposon
variants carrying synthetic or wild-type termini but no intact Tn1000 genes. Transposon …

Escherichia coli RNA polymerase binding sites and transcription initiation sites in the transposon Tn3

WL Wishart, M Chiyoko, O Hisako, O Eiichi - Gene, 1983 - Elsevier
We have identified the Escherichia coli RNA polymerase-binding sites and the transcription
initiation sites in the transposon Tn3. Results from nitrocellulose filter-binding assays …