The role of the interdomain B linker in the activation of the XylR protein of Pseudomonas putida

J Garmendia, V De Lorenzo - Molecular microbiology, 2000 - Wiley Online Library
In the presence of toluene and other structural analogues, the enhancer binding protein
XylR activates the σ54 promoter Pu of the TOL (toluene degradation) plasmid pWW0 of …

Activation and Repression of Transcription at the Double Tandem Divergent Promoters for the xylR and xylS Genes of the TOL Plasmid of Pseudomonas putida

S Marqués, MT Gallegos, M Manzanera… - Journal of …, 1998 - Am Soc Microbiol
The xylR and xylS genes are divergent and control transcription of the TOL plasmid
catabolic pathways for toluene metabolism. Four promoters are found in the 300-bp …

Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70-and alpha 54-dependent tandem …

MT Gallegos, S Marqués, JL Ramos - Journal of bacteriology, 1996 - Am Soc Microbiol
Growth of Pseudomonas putida (pWWO) on alkylbenzoates requires the expression of the
meta pathway operon, which is mediated by the XylS protein after binding of a benzoate …

[HTML][HTML] In Vivo UV Laser Footprinting of thePseudomonas putida ς54PuPromoter Reveals That Integration Host Factor Couples Transcriptional Activity to Growth …

M Valls, M Buckle, V de Lorenzo - Journal of Biological Chemistry, 2002 - ASBMB
The occupation of the ς 54-dependent Pu promoter of Pseudomonas putida by the
integration host factor (IHF) under different growth conditions has been monitored in its …

Involvement of IHF protein in expression of the Ps promoter of the Pseudomonas putida TOL plasmid

A Holtel, D Goldenberg, H Giladi… - Journal of …, 1995 - Am Soc Microbiol
Regulation of the xyl gene operons of the Pseudomonas putida TOL plasmid is mediated by
the products of the downstream clustered and divergently oriented xylR and xylS regulatory …

Functional analysis of the integration host factor site of the σ54Pu promoter of Pseudomonas putida by in vivo UV imprinting

M Valls, R Silva‐Rocha, I Cases, A Muñoz… - Molecular …, 2011 - Wiley Online Library
The integration host factor (IHF) of Pseudomonas putida connects cell growth to
transcriptional activity of distinct promoters. The IHF site of the σ54 promoter Pu of the TOL …

Genetic evidence of separate repressor and activator activities of the XylR regulator of the TOL plasmid, pWWO, of Pseudomonas putida

G Bertoni, J Perez‐Martfn… - Molecular …, 1997 - Wiley Online Library
The XylR protein encoded by pWWO, the TOL (toluene biodegradation) plasmid of
Pseudomonas putida, activates at a distance the transcription of Pu and Ps, which are the …

[HTML][HTML] CRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive σ54-Promoter Pu in Escherichia coli

YT Zhang, F Jiang, ZX Tian, YX Huo, YC Sun… - PLoS …, 2014 - journals.plos.org
The expression of σ54-dependent Pseudomonas putida Pu promoter is activated by XylR
activator when cells are exposed to a variety of aromatic inducers. In this study, the …

Cooperative amino acid changes shift the response of the σ54‐dependent regulator XylR from natural m‐xylene towards xenobiotic 2,4‐dinitrotoluene

A de Las Heras, V de Lorenzo - Molecular microbiology, 2011 - Wiley Online Library
XylR is a σ54‐dependent transcriptional factor of Pseudomonas putida that activates the Pu
promoter of the TOL plasmid upon binding its natural effector, m‐xylene. The search for …

Roles of effectors in XylS-dependent transcription activation: intramolecular domain derepression and DNA binding

P Domínguez-Cuevas, P Marín, S Busby… - Journal of …, 2008 - Am Soc Microbiol
XylS, an AraC family protein, activates transcription from the benzoate degradation pathway
Pm promoter in the presence of a substrate effector such as 3-methylbenzoate (3MB). We …