Physical organization of the upper pathway operon promoter of the Pseudomonas TOL plasmid. Sequence and positional requirements for XylR-dependent activation …

MA Abril, JL Ramos - Molecular and General Genetics MGG, 1993 - Springer
The upper pathway operon of the Pseudomonas putida TOL plasmid belongs to the− 12/-24
class of promoters. These promoters exhibit three regions critical for regulated transcription …

The TACAN4TGCA motif upstream from the-35 region in the sigma70-sigmaS-dependent Pm promoter of the TOL plasmid is the minimum DNA segment required for …

MT Gallegos, S Marqués, JL Ramos - Journal of bacteriology, 1996 - Am Soc Microbiol
Transcription from the TOL plasmid meta-cleavage pathway operon promoter Pm is
dependent on the XylS regulator activated by benzoate effectors or after XylS …

Coactivation in vitro of the sigma54-dependent promoter Pu of the TOL plasmid of Pseudomonas putida by HU and the mammalian HMG-1 protein

J Pérez-Martín, V De Lorenzo - Journal of bacteriology, 1997 - Am Soc Microbiol
The mechanism by which the prokaryotic histone-like protein HU replaces the integration
host factor (IHF) in the coactivation of the sigma54-dependent promoter Pu of Pseudomonas …

The XylS‐dependent Pm promoter is transcribed in vivo by RNA polymerase with σ32 or σ38 depending on the growth phase

S Marqués, M Manzanera… - Molecular …, 1999 - Wiley Online Library
The Pm promoter of the TOL plasmid of Pseudomonas putida is expressed at high level
along the growth curve. This transcription is dependent on the positive regulator XylS …

[HTML][HTML] Single Amino Acids Changes in the Signal Receptor Domain of XylR Resulted in Mutants That Stimulate Transcription in the Absence of Effectors (∗)

A Delgado, R Salto, S Marqués, JL Ramos - Journal of Biological Chemistry, 1995 - ASBMB
The XylR protein positively controls expression from the Pseudomonas putida TOL plasmid
σ 54-dependent" upper" pathway operon promoter (Pu) and the xylS gene promoter (Ps), in …

Modulation of the function of the signal receptor domain of XylR, a member of a family of prokaryotic enhancer-like positive regulators

R Salto, A Delgado, C Michán, S Marqués… - Journal of …, 1998 - Am Soc Microbiol
The XylR protein controls expression from the Pseudomonas putida TOL plasmid upper
pathway operon promoter (Pu) in response to aromatic effectors. XylR-dependent …

In vivo drafting of single‐chain antibodies for regulatory duty on the σ54‐promoter Pu of the TOL plasmid

P Jurado, LA Fernández… - Molecular …, 2006 - Wiley Online Library
The identification of single‐chain antibodies (scFvs) that interfere in vivo with the building of
the complex that activate the prokaryotic, σ54‐dependent promoter Pu of the catabolic TOL …

[HTML][HTML] Active recruitment of σ54-RNA polymerase to the Pu promoter of Pseudomonas putida: role of IHF and αCTD

G Bertoni, N Fujita, A Ishihama, V de Lorenzo - The EMBO journal, 1998 - embopress.org
The sequence elements determining the binding of the σ 54-containing RNA polymerase (σ
54-RNAP) to the Pu promoter of Pseudomonas putida have been examined. Contrary to …

Monitoring Intracellular Levels of XylR in Pseudomonas putida with a Single-Chain Antibody Specific for Aromatic-Responsive Enhancer-Binding Proteins

S Fraile, F Roncal, LA Fernández… - Journal of …, 2001 - Am Soc Microbiol
We have isolated a recombinant phage antibody (Phab) that binds a distinct epitope of the
subclass of the ς54-dependent prokaryotic enhancer-binding proteins that respond directly …

Transcriptional wiring of the TOL plasmid regulatory network to its host involves the submission of the σ54‐promoter Pu to the response regulator PprA

E Vitale, A Milani, F Renzi, E Galli… - Molecular …, 2008 - Wiley Online Library
Implantation of the regulatory circuit of the degradation pathway of TOL plasmid pWW0 in
the native transcriptional network of the host Pseudomonas putida involves interplay …