The development of methods for cell‐mediated transgenesis, based on somatic cell nuclear
transfer, provides a tremendous opportunity to shape the genetic make‐up of livestock …

Until recently genetically modified livestock could only be generated by pronuclear injection.
The discovery that animals can be cloned by nuclear transfer from cultured somatic cells …

Until recently, precise modification of the animal genome by gene targeting was restricted to
the mouse because germline competent embryonic stem cells are not available in any other …

The technology of gene targeting through homologous recombination has been extremely
useful for elucidating gene functions in mice. The application of this technology was thought …

Gene transfer to animal cells was first achieved more than thirty years ago. Since then,
transformation technology has developed rapidly, resulting in a multitude of techniques for …

In contrast to the highly developed genetic modification systems available for manipulating
the mouse genome, at this time only simple gain of function modifications can be undertaken …

Contents Somatic cell nuclear transfer (SCNT) was first developed in livestock for the
purpose of accelerating the widespread use of superior genotypes. Although many …

The birth and adult development of “Dolly” the sheep, the first mammal produced by transfer
of a terminally differentiated cell nucleus into an egg, provided unequivocal evidence of …

Genome editing tools enable efficient and accurate genome manipulation. An enhanced
ability to modify the genomes of livestock species could be utilized to improve disease …

Restoring nuclear totipotency in differentiated somatic cells following nuclear transfer, to
produce healthy cloned animals, remains remarkable but highly inefficient and prone to …