Main conclusion Studies on the tissue culture of apple have allowed for molecular,
biotechnological and applied breeding research to advance. In the past 8 years, over 100 …

Abstract Apple (Malus domestica Borkh.), which is a widely cultivated, important economic
fruit crop with nutritive and medicinal importance, has emerged as a model horticultural crop …

Abstract Background Wild apple, Malus sieversii, is an endangered species and a valuable
genetic resource that requires a variety of conservation techniques. This study aimed to …

For micropropagation of adult apple (Malus domestica Borkh) rootstock M9, explants were
harvested from pre-chilled (4±3° C) dormant cuttings forced in growth chamber. Primary …

Effective regeneration in vitro is a necessary precondition for the implementation of different
biotechnological approaches in plant breeding. Numerous studies have reported on …

Key message Cryopreservation provides valuable genes for further breeding of elite
cultivars, and cryotherapy improves the production of virus-free plants in Malus spp., thus …

Initiation of shoot cultures from field-grown plants is often difficult due to the high level of
bacterial and fungal organisms that populate trees. This study compared shoot initiation …

A simple and efficient cryopreservation protocol using encapsulation-dehydration was
established for in vitro-grown shoot-tips of apple 'Gala'(Malus× domestica Borkh.). Shoot …

Apple shoot meristems have been cultured aseptically to promote growth and proliferation of
shoots. Apices grown in a modified LS medium with cytokinin but in the absence of auxin …

Here, we report an efficient and widely applicable method for cryopreservation of Malus
shoot tips by encapsulation–dehydration using adventitious shoots. Shoots were induced …