BEAR reveals that increased fidelity variants can successfully reduce the mismatch tolerance of adenine but not cytosine base editors
Adenine and cytosine base editors (ABE, CBE) allow for precision genome engineering.
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …
Here, Base Editor Activity Reporter (BEAR), a plasmid-based fluorescent tool is introduced …
A Cas9-mediated adenosine transient reporter enables enrichment of ABE-targeted cells
N Brookhouser, T Nguyen, SJ Tekel, K Standage-Beier… - BMC biology, 2020 - Springer
Background Adenine base editors (ABE) enable single nucleotide modifications without the
need for double-stranded DNA breaks (DSBs) induced by conventional CRIPSR/Cas9 …
need for double-stranded DNA breaks (DSBs) induced by conventional CRIPSR/Cas9 …
nCas9 Engineering for Improved Target Interaction Presents an Effective Strategy to Enhance Base Editing
G Zhang, Z Song, S Huang, Y Wang, J Sun… - Advanced …, 2024 - Wiley Online Library
Base editors (BEs) are a recent generation of genome editing tools that couple a cytidine or
adenosine deaminase activity to a catalytically impaired Cas9 moiety (nCas9) to enable …
adenosine deaminase activity to a catalytically impaired Cas9 moiety (nCas9) to enable …
Expanding the genome-targeting scope and the site selectivity of high-precision base editors
Base editors (BEs) are RNA-guided CRISPR-Cas-derived genome editing tools that induce
single-nucleotide changes. The limitations of current BEs lie in their low precision …
single-nucleotide changes. The limitations of current BEs lie in their low precision …
A new compact adenine base editor generated through deletion of HNH and REC2 domain of SpCas9
Y Qian, D Wang, W Niu, D Zhao, J Li, Z Liu, X Gao… - BMC biology, 2023 - Springer
Abstract Background Adenine base editors (ABEs) are promising therapeutic gene editing
tools that can efficiently convert targeted A• T to G• C base pairs in the genome. However …
tools that can efficiently convert targeted A• T to G• C base pairs in the genome. However …
A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects
Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their
DNA and RNA off-target activities have caused concerns in biomedical applications. We …
DNA and RNA off-target activities have caused concerns in biomedical applications. We …
Hyperactive Nickase Activity Improves Adenine Base Editing
AP Gandadireja, PD Vos, SJ Siira… - ACS Synthetic …, 2024 - ACS Publications
Base editing technologies enable programmable single-nucleotide changes in target DNA
without double-stranded DNA breaks. Adenine base editors (ABEs) allow precise …
without double-stranded DNA breaks. Adenine base editors (ABEs) allow precise …
Circularly permuted and PAM-modified Cas9 variants broaden the targeting scope of base editors
Base editing requires that the target sequence satisfy the protospacer adjacent motif
requirement of the Cas9 domain and that the target nucleotide be located within the editing …
requirement of the Cas9 domain and that the target nucleotide be located within the editing …
Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity
Applications of adenine base editors (ABEs) have been constrained by the limited
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …
compatibility of the deoxyadenosine deaminase component with Cas homologs other than …
Cas9 variants expand the targeting scope of base editing systems in bacteria
RM Shelake, JY Kim - The Nucleus, 2024 - Springer
Base editors (BEs) consist of partially active Cas9 homologs fused to nucleobase-converting
DNA deaminases along with certain accessory proteins. The effectiveness of BE tools …
DNA deaminases along with certain accessory proteins. The effectiveness of BE tools …