We used quantitative complementation assays to characterize individual DNA polymerase β
(Pol β) mutants for their ability to function in DNA replication and DNA repair. We also …

DNA polymerase β (pol β) is a 39-kDa protein that functions in DNA repair processes in
mammalian cells. As a first step toward understanding mechanisms of polymerase fidelity …

We have used random sequence mutagenesis to generate mutants of DNA polymerase β in
an effort to identify amino acid residues important for function, catalytic efficiency and fidelity …

We have designed and utilized a bacterial complementation system to identify and
characterize mammalian DNA polymerase beta mutants. In this complementation system …

DNA polymerase lambda (pol λ) is a novel family X DNA polymerase that has been
suggested to play a role in meiotic recombination and DNA repair. The recent demonstration …

DNA polymerase β functions in both base excision repair and meiosis. Errors committed by
polymerase β during these processes could result in mutations. Using a complementation …

Differences in the substrate specificity of mammalian family X DNA polymerases are
proposed to partly depend on a loop (loop 1) upstream of the polymerase active site. To …

Overexpression in mammalian cells of the error-prone DNA polymerase β (Pol β) has been
found to increase the spontaneous mutagenesis. Here, we investigated a possible …

DNA polymerase δ (Pol δ) plays a central role in eukaryotic chromosomal DNA replication,
repair and recombination1. Highly purified preparations of mammalian Pol δ typically …

Department of Chemistry, The PennsylvaniaState University, University Park, Pennsylvania
16802 Received July 11, 1990; Revised Manuscript Received September 19, 1990 abstract …