[HTML][HTML] Efficient gene targeting in maize using inducible CRISPR-Cas9 and marker-free donor template
CRISPR-Cas9 is a powerful tool for generating targeted mutations and genomic deletions.
However, precise gene insertion or sequence replacement remains a major hurdle before …
However, precise gene insertion or sequence replacement remains a major hurdle before …
Single and multiple gene knockouts by CRISPR–Cas9 in maize
NM Doll, LM Gilles, MF Gérentes, C Richard, J Just… - Plant Cell Reports, 2019 - Springer
Key message The analysis of 93 mutant alleles in 18 genes demonstrated that CRISPR–
Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of …
Cas9 is a robust tool for targeted mutagenesis in maize, permitting efficient generation of …
CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination
Key message We summarize recent progress of CRISPR/Cas9-mediated gene targeting in
plants, provide recommendations for designing gene-targeting vectors and highlight the …
plants, provide recommendations for designing gene-targeting vectors and highlight the …
[HTML][HTML] Targeted genome modifications in soybean with CRISPR/Cas9
Background The ability to selectively alter genomic DNA sequences in vivo is a powerful tool
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …
for basic and applied research. The CRISPR/Cas9 system precisely mutates DNA …
Advances in Agrobacterium transformation and vector design result in high‐frequency targeted gene insertion in maize
D Peterson, P Barone, B Lenderts… - Plant Biotechnology …, 2021 - Wiley Online Library
CRISPR‐Cas is a powerful DNA double‐strand break technology with wide‐ranging
applications in plant genome modification. However, the efficiency of genome editing …
applications in plant genome modification. However, the efficiency of genome editing …
[HTML][HTML] Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes
S Svitashev, C Schwartz, B Lenderts, JK Young… - Nature …, 2016 - nature.com
Targeted DNA double-strand breaks have been shown to significantly increase the
frequency and precision of genome editing. In the past two decades, several double-strand …
frequency and precision of genome editing. In the past two decades, several double-strand …
Efficiency and inheritance of targeted mutagenesis in maize using CRISPR-Cas9
CRISPR/Cas (clustered regularly interspaced short palindromic repeats/CRISPR-associated
proteins) is an adaptive immune system in bacteria and archaea to defend against invasion …
proteins) is an adaptive immune system in bacteria and archaea to defend against invasion …
An Agrobacterium‐delivered CRISPR/Cas9 system for high‐frequency targeted mutagenesis in maize
SN Char, AK Neelakandan… - Plant biotechnology …, 2017 - Wiley Online Library
CRISPR/Cas9 is a powerful genome editing tool in many organisms, including a number of
monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler …
monocots and dicots. Although the design and application of CRISPR/Cas9 is simpler …
Heat‐shock‐inducible CRISPR/Cas9 system generates heritable mutations in rice
Transient expression of CRISPR/Cas9 is an effective approach for limiting its activities and
improving its precision in genome editing. Here, we describe the heat‐shock‐inducible …
improving its precision in genome editing. Here, we describe the heat‐shock‐inducible …
Activities and specificities of CRISPR/Cas9 and Cas12a nucleases for targeted mutagenesis in maize
CRISPR/Cas9 and Cas12a (Cpf1) nucleases are two of the most powerful genome editing
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …
tools in plants. In this work, we compared their activities by targeting maize glossy2 gene …