Identification of shiga toxin-producing bacteria by a new immuno-capture toxin gene PCR

W Luo, S Wang, X Peng - FEMS microbiology letters, 2002 - academic.oup.com
Infections with bacteria producing shiga toxin are responsible for widespread disease and
for the death of a large number of people. In the present study, we have developed a rapid …

A method for detecting Shiga toxin and Shiga-like toxin-l in pure and mixed culture

S Ashkenazi, TG Cleary - Journal of medical microbiology, 1990 - microbiologyresearch.org
Shiga toxin and Shiga-like toxins (SLTs, syn. Verotoxins) are currently detected by tissue
culture assays that are expensive, time-consuming and require specialised facilities and …

Enzyme-linked immunosorbent assay for shigella toxin

A Donohue-Rolfe, MA Kelley, M Bennish… - Journal of clinical …, 1986 - Am Soc Microbiol
An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of
shigella toxin. For the assay, a mouse monoclonal antibody against the B subunit of the toxin …

Detection and Subtyping of ShigaToxin-Producing Escherichia coli (STEC)

P Gallien - PCR Detection of Microbial Pathogens, 2003 - Springer
Abstract When the Japanese microbiologist Shiga discovered a bacterium causing
dysentery in humans in 1898, the organism was designated Shigella dysenteriae type 1 …

Enzyme-linked immunosorbent assay to detect Shiga toxin of Shigella dysenteriae and related toxins

U Kongmuang, T Honda, T Miwatani - Journal of clinical …, 1987 - Am Soc Microbiol
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed for detection of
Shiga toxin. Four species of Shigella, Escherichia coli, and Vibro parahaemolyticus were …

Detection of Shiga toxin-producing Shigella dysenteriae type 1 and Escherichia coli by using polymerase chain reaction with incorporation of digoxigenin-11-dUTP

MP Jackson - Journal of Clinical Microbiology, 1991 - Am Soc Microbiol
A technique has been developed for the detection of Shiga toxin-and Shiga-like toxin type I
(ShT/SLT-I)-producing Shigella dysenteriae type 1 and Escherichia coli by using the …

DNA probes to identify Shiga-like toxin I-and II-producing enteric bacterial pathogens isolated from patients with diarrhea in Thailand

J Seriwatana, JE Brown, P Echeverria… - Journal of clinical …, 1988 - Am Soc Microbiol
When Shigella species, Escherichia coli, and five other bacterial enteric pathogens isolated
from children with diarrhea in Thailand were tested for hybridization under stringent …

Identification of Shiga toxins in Shiga toxin-producing Escherichia coli using immunoprecipitation and high-performance liquid chromatography-electrospray ionization …

F Kondo, H Saito, R Hayashi, H Onda, S Kobayashi… - Analyst, 2003 - pubs.rsc.org
We developed a rapid and reliable identification method for Shiga toxins in Shiga toxin-
producing Escherichia coli (STEC) using immunoprecipitation and high-performance liquid …

Sensitive detection of Shiga Toxin 2 and some of its variants in environmental samples by a novel immuno-PCR assay

X He, W Qi, B Quiñones, S McMahon… - Applied and …, 2011 - Am Soc Microbiol
Shiga toxin-producing Escherichia coli (STEC) in the environment has been reported
frequently. However, robust detection of STEC in environmental samples remains difficult …

Evaluation of the Alere SHIGA TOXIN QUIK CHEK™ in comparison to multiplex Shiga toxin PCR

K De Rauw, J Breynaert, D Piérard - Diagnostic Microbiology and Infectious …, 2016 - Elsevier
Shiga toxin-producing Escherichia coli (STEC) causes gastrointestinal outbreaks worldwide
and due to its low infectious dose highly sensitive diagnostics are needed. In this study, the …