Selection has been invaluable for genetic manipulation, although counter-selection has
historically exhibited limited robustness and convenience. TolC, an outer membrane pore …

Engineering of bacterial genomes is a fundamental craft in contemporary biotechnology.
The ability to precisely edit chromosomes allows for the development of cells with specific …

Oligonucleotide-mediated multiplex genome engineering is an important tool for bacterial
genome editing. The efficient application of this technique requires the inactivation of the …

We present a method for identifying genomic modifications that optimize a complex
phenotype through multiplex genome engineering and predictive modeling. We apply our …

Background Gene editing is key for elucidating gene function. Traditional methods, such as
consecutive single-crossovers, have been widely used to modify bacterial genomes …

Currently available tools for multiplex bacterial genome engineering are optimized for a few
laboratory model strains, demand extensive prior modification of the host strain, and lead to …

The removal of unwanted genetic material is a key aspect in many synthetic biology efforts
and often requires preliminary knowledge of which genomic regions are dispensable …

Genome engineering using single-stranded oligonucleotides is an efficient method for
generating small chromosomal and episomal modifications in a variety of host organisms …

Precision genome editing accelerates the discovery of the genetic determinants of
phenotype and the engineering of novel behaviors in organisms. Advances in DNA …

Advances in genetic manipulation and genome engineering techniques have enabled on-
demand targeted deletion, insertion, and substitution of DNA sequences. One important step …