We show that relaxase TrwC promotes recombination between two directly repeated oriTs
while related relaxases TraI of F and pKM101 do not. Efficient recombination required also …

ABSTRACT R388 conjugative relaxase TrwC acts as a site-specific recombinase, promoting
recombination between two cognate oriT s on double-stranded DNA substrates. The …

Conjugative relaxases are the proteins that initiate bacterial conjugation by a site-specific
cleavage of the transferred DNA strand. In vitro, they show strand-transferase activity on …

Relaxosomes are specific nucleoprotein structures involved in DNA‐processing reactions
during bacterial conjugation. In this work, we present evidence indicating that plasmid R388 …

Plasmids containing a direct repeat of plasmid R388 oriT are capable of site-specific
recombination, which results in deletion of the intervening DNA. This reaction occurs in the …

The conjugative transfer region of the IncX plasmid R6K (TRAX) was analysed by
transposon mutagenesis and DNA sequencing. Tn5tac1 insertional mutations localized …

Background Bacterial conjugation is a mechanism for horizontal DNA transfer between
bacteria which requires cell to cell contact, usually mediated by self-transmissible plasmids …

The mobilization region of plasmid CloDF13 was localized to a 3.6 kb DNA segment that
was analysed by transposon mutagenesis and DNA sequencing. Analysis of the DNA …

The plasmids R1162 and pSC101 have origins of conjugative transfer (oriT s) and
corresponding relaxases that are closely related. The oriT s are made up of a highly …

Bacterial conjugation is a mechanism of horizontal DNA transfer. The relaxase TrwC of the
conjugative plasmid R388 cleaves one strand of the transferred DNA at the oriT gene …