TrwA protein was purified from an overproducing Escherichia coli strain and characterized
as a 53 kDa tetrameric DNA-binding protein. Gel shift assays showed that TrwA bound …

Protein TrwC is the relaxase-helicase responsible for the initiation and termination reactions
of DNA processing during plasmid R388 conjugation. Site-directed mutagenesis was used …

Relaxases act as DNA selection sieves in conjugative plasmid transfer. Most plasmid
relaxases belong to the HUH endonuclease family. TrwC, the relaxase of plasmid R388, is …

Type IV secretion system (T4SS) substrates are recruited through a translocation signal that
is poorly defined for conjugative relaxases. The relaxase TrwC of plasmid R388 is …

Orf20 of the conjugative transposon Tn 916 was purified as a chimeric protein fused to
maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic …

Transfer of plasmid RP4 during bacterial conjugation requires the plasmid-encoded TraJ
protein, which binds to the transfer origin (Fürste, JP, Pansegrau, W., Ziegelin, G., Kröger, M …

F plasmid‐mediated bacterial conjugation requires interactions between a relaxosome
component, TraM, and the coupling protein TraD, a hexameric ring ATPase that forms the …

Antibiotic resistance is a serious global problem. Antibiotic resistance genes (ARG), which
are widespread in environmental bacteria, can be transferred to pathogenic bacteria via …

Genetic and biochemical characterization of TraA, the relaxase of symbiotic plasmid
pRetCFN42d from Rhizobium etli, is described. After purifying the relaxase domain …

Bacterial conjugation is a promiscuous DNA transport mechanism. Conjugative plasmids
transfer themselves between most bacteria, thus being one of the main causal agents of the …