Highly purified poly (A) polymerase (polynucleotide adenylyltransferase, EC 2.7. 7.19),
which synthesizes poly (A) from ATP substrate, can also catalyze hydrolysis of poly (A). The …

Publisher Summary This chapter discusses the multiplicity, cellular localization, and
regulation of poly (A) adding enzymes. It illustrates the poly (A) adding reactions that are …

Poly (A) polymerase activity was first detected in yeast extracts, primarily in association with
the ribosomal fraction, by Twu and Bretthauer in 1971 (Twu, JS, and Bretthauer, RK.(1971) …

cDNA clones encoding mammalian poly (A) polymerase were isolated with probes
generated by the polymerase chain reaction based on amino acid sequences derived from …

Bovine poly (A) polymerase was purified from overexpressing strains of Escherichia coli and
from Spodoptera frugiperda Sf21 cells infected with a recombinant baculovirus. The E. coli …

To determine the role of poly (A) polymerase in 3′-end processing of mRNA, the effect of
purified poly (A) polymerase antibodies on endonucleolytic cleavage and polyadenylation …

Publisher Summary This chapter discusses the enzymes that are likely to be responsible for
synthesis of poly (A) sequences and has led to new methods for obtaining preparations of …

Previous studies have demonstrated that poly (A) polymerase exists as “bound” and “free”
forms localized in the chromatin and nuclear supernatant (sap) fractions, respectively …

Polyadenylate sequences of 100 to 200 AMP residues can be covalently attached to the 3′
hydroxyl group of several polyribonucleotides, but not to deoxypolyribonucleotides, with a …

In the cell nucleus, poly (A) polymerase (EC 2.7. 7.19) very probably catalyzes the non-
transcriptive addition of poly (A) to hnRNA-molecules assumed to be the precursors to …