Aims: The enzymatic dissociation of pancreatic tissue by collagenase is a key step in the
islet isolation procedure. Previous investigations clearly showed that purified collagenase …

BACKGROUND: Islet release from the pancreas is mediated by both collagenase and
neutral protease (NP), a critical effector of islet integrity. To prove the hypothesis that …

Aims: The enzymatic dissociation of pancreatic tissue by collagenase is a key step in the
islet isolation procedure. Previous investigations in the rat clearly showed that neutral …

Background. Islet isolation from the pancreatic tissue matrix remains highly variable. Recent
evidence suggests that intrinsic human pancreatic proteases, including trypsin, may inhibit …

Background. The introduction of the two-layer method (TLM) for long-term human pancreas
preservation revealed the enormous potential of TLM to improve graft function of isolated …

Background. We have reported improved islet isolation outcomes using a new digestion
protocol where the pancreas is perfused only with collagenase, and neutral protease (NP) is …

Aims: The oxygenation of the human pancreas during cold storage was recently established
for clinical islet transplantation by introduction of the two-layer method (TLM). Nevertheless …

Introduction. Most manufacturers provide collagenase and noncollagenolytic enzyme in
separate vials, as opposed to previous products containing both collagenase and non …

Aims: The enzymatic dissociation of pancreatic tissue by collagenase is a key step in the
islet isolation procedure. Previous investigations clearly showed that the successful release …

Background. The isolation of islets from the human pancreas critically depends on an
efficient enzyme blend. Previous studies have solely focused on the presence of …