Characterization of the interactions between soluble factors required for nuclear transport is
key to understanding the process of nuclear trafficking. Using a synthetic lethal screen with …

Many nuclear proteins are imported into the cell nucleus by the “classical” nuclear
localization signal (NLS)-mediated import pathway. In this pathway, a sequence rich in basic …

We have purified a cytosolic protein from Xenopus eggs that is essential for selective protein
import into the cell nucleus. The purified protein, named importin, promotes signal …

We have used in vitro binding assays to examine specific interactions between a number of
cytoplasmic and nuclear pore proteins involved in nuclear protein import in vertebrates. We …

After synthesis in the cytoplasm, nuclear proteins traverse the nuclear envelope as a result
of the specific recognition of nuclear localization signals by import. Various approaches …

THE import of nuclear proteins proceeds through the nuclear pore complex and requires
nuclear localization signals (NLSs) 1, 2, energy3, 4 and soluble factors5, namely importin-α …

Proteins containing a classical NLS are transported into the nucleus by the import receptor
importin β, which binds to cargoes via the adaptor importin α. The import complex is …

Cargos destined to enter or leave the cell nucleus are typically transported by receptors of
the importin β family to pass the nuclear pore complex. The yeast Saccharomyces cerevisiae …

The trafficking of protein and RNA cargoes between the cytoplasm and the nucleus of
eukaryotic cells, which is a major pathway involved in cell regulation, is mediated by nuclear …

The complex of importin‐alpha and‐beta is essential for nuclear protein import. It binds the
import substrate in the cytosol, and the resulting trimeric complex moves through the nuclear …