Effects of sperm pretreatment on efficiency of ICSI-mediated gene transfer in pigs

M Kurome, H Saito, R Tomii, S Ueno… - … of Reproduction and …, 2007 - jstage.jst.go.jp
Intracytoplasmic sperm injection (ICSI)-mediated gene transfer has recently been shown to
be an effective technique for producing transgenic pigs; however, the types of sperm …

Progress and emerging problems in livestock transgenesis: a summary perspective

RF Seamark - Reproduction, fertility and development, 1994 - CSIRO Publishing
The creation of transgenic livestock is a complex multistep procedure the successful
execution of which demands a high level of skill and application. Useful animals have been …

[PDF][PDF] Gene expression during pre-and peri-implantation embryonic development in pigs

P Maddox-Hyttel, A Dinnyes, J Laurincik… - REPRODUCTION …, 2001 - researchgate.net
Embryo technological procedures such as in vitro production and cloning by nuclear transfer
are not as advanced in pigs as in cattle and cannot yet be applied under field conditions …

Early embryonic development, assisted reproductive technologies, and pluripotent stem cell biology in domestic mammals

V Hall, K Hinrichs, G Lazzari, DH Betts, P Hyttel - The Veterinary Journal, 2013 - Elsevier
Over many decades assisted reproductive technologies, including artificial insemination,
embryo transfer, in vitro production (IVP) of embryos, cloning by somatic cell nuclear transfer …

Characterization of the ICSI‐mediated gene transfer method in the production of transgenic pigs

K Umeyama, H Saito, M Kurome… - Molecular …, 2012 - Wiley Online Library
Understanding the behavior of transgenes introduced into oocytes or embryos is essential
for evaluating the methodologies for transgenic animal production. We investigated the …

In vitro production of (Farm) animal embryos

C Wrenzycki - Animal Biotechnology 1: Reproductive Biotechnologies, 2018 - Springer
Over the past decades, in vitro production (IVP) of bovine embryos has been significantly
improved, and in particular bovine IVP is now widely applied under field conditions. This in …