The recent development of numerous technologies for proteome analysis holds the promise
of new and more precise methods for disease diagnosis. In this review, we provide an …

Gel-based proteomics is one of the most versatile methods for fractionating protein
complexes. Among these methods, two dimensional-polyacrylamide gel electrophoresis (2 …

Proteomics has traditionnally used the separating power of two‐dimensional electrophoresis
for the quantitative analysis of protein amounts in complex extracts. However, the limitations …

The last few years have seen an unprecedented drive toward the application of proteomics
to resolving challenging biomedical and biochemical tasks. Separation techniques …

Proteomic research has proved valuable for understanding the molecular mechanisms of
biological processes, as well as in the search for biomarkers for a variety of diseases which …

The high resolving power of two-dimensional polyacrylamide gel electrophoresis 2D-PAGE
and its full analytical and preparative potential have been described with special emphasis …

Publisher Summary The chapter focuses on the current strategies for quantitative
proteomics. The establishment of an absolute quantitation requires the use of internal …

Significant technological advances in protein chemistry, physics and computer sciences in
the last two decades have greatly improved protein separation methodologies, such as …

Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key
components of current proteomics technology. The purpose of this review is to give an …

Abstract Two-dimensional gel electrophoresis (2-DE) is a technique that has been widely
applied in a variety of proteomics studies. It is capable of resolving complex protein mixtures …