The Marburg Collection: A Golden Gate DNA Assembly Framework for Synthetic Biology Applications in Vibrio natriegens

D Stukenberg, T Hensel, J Hoff, B Daniel… - ACS synthetic …, 2021 - ACS Publications
Vibrio natriegens is known as the world's fastest growing organism with a doubling time of
less than 10 min. This incredible growth speed empowers V. natriegens as a chassis for …

Vibrio natriegens, a new genomic powerhouse

HH Lee, N Ostrov, BG Wong, MA Gold, AS Khalil… - BioRxiv, 2016 - biorxiv.org
Recombinant DNA technology has revolutionized biomedical research with continual
innovations advancing the speed and throughput of molecular biology. Nearly all these …

Efficient natural plasmid transformation of Vibrio natriegens enables zero-capital molecular biology

DA Specht, TJ Sheppard, F Kennedy, S Li… - PNAS …, 2024 - academic.oup.com
The fast-growing microbe Vibrio natriegens is capable of natural transformation where it
draws DNA in from media via an active process under physiological conditions. Using an …

Synthetic Biology Tools for the Fast-Growing Marine Bacterium Vibrio natriegens

T Tschirhart, V Shukla, EE Kelly… - ACS synthetic …, 2019 - ACS Publications
The fast-growing nonmodel marine bacterium Vibrio natriegens has recently garnered
attention as a host for molecular biology and biotechnology applications. In order to further …

Design and Reconstruction of Regulatory Parts for Fast-Growing Vibrio natriegens Synthetic Biology

F Wu, W Chen, Y Peng, R Tu, Y Lin, J Xing… - ACS Synthetic …, 2020 - ACS Publications
The fast-growing Vibrio natriegens is an attractive robust chassis for diverse synthetic
biology applications. However, V. natriegens lacks the suitable constitutive regulatory parts …

Establishing a Cell-Free Vibrio natriegens Expression System

DJ Wiegand, HH Lee, N Ostrov… - ACS synthetic biology, 2018 - ACS Publications
The fast growing bacterium Vibrio natriegens is an emerging microbial host for
biotechnology. Harnessing its productive cellular components may offer a compelling …

[HTML][HTML] Identification and cross-characterisation of artificial promoters and 5′ untranslated regions in Vibrio natriegens

L Tietze, A Mangold, MW Hoff, R Lale - Frontiers in Bioengineering …, 2022 - frontiersin.org
Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in
synthetic biology applications. Currently, a limited set of genetic elements optimised for …

Vibrio natriegens: an ultrafast‐growing marine bacterium as emerging synthetic biology chassis

J Hoff, B Daniel, D Stukenberg… - Environmental …, 2020 - Wiley Online Library
The marine bacterium Vibrio natriegens is the fastest‐growing non‐pathogenic bacterium
known to date and is gaining more and more attention as an alternative chassis organism to …

Recombinant Protein Expression Chassis Library of Vibrio natriegens by Fine-Tuning the Expression of T7 RNA Polymerase

Y Sun, J Xu, H Zhou, H Zhang, J Wu… - ACS Synthetic …, 2023 - ACS Publications
Vibrio natriegens is the fastest-growing bacteria, and its doubling time is less than 10 min. At
present, the T7 expression system has been introduced into V. natriegens for heterologous …

Vibrio natriegens as a fast-growing host for molecular biology

MT Weinstock, ED Hesek, CM Wilson, DG Gibson - Nature methods, 2016 - nature.com
A rapidly growing bacterial host would be desirable for a range of routine applications in
molecular biology and biotechnology. The bacterium Vibrio natriegens has the fastest …