Efficient model production in rats that incorporates newly developed genetic editing and
embryo transfer tools, such as CRISPR/Cas9 technology and non-surgical embryo transfer …

The rat is widely used as a laboratory animal for research. In particular, genetically
engineered rats are essential for production of animal models of several diseases. Although …

A high potassium concentration in culture media is considered detrimental to in vitro culture
of mouse embryos. Here we show that pig zygotic medium (PZM) containing a higher …

A modified KSOM for rat embryo culture (KSOM-R), which has enriched taurine, glycine,
glutamic acid, and alanine, promoted rat embryo development in vitro. Since mice and rats …

In vitro development of rabbit embryos was investigated. Zygotes recovered from female
oviducts at the pronuclear stage (19-22 hpc) were cultured in vitro at standard temperature …

One-cell embryos from outbred mice (CF1, CD-1, and Dub: ICR) were cultured in various
modifications of egg culture medium (ECM). The best development was observed in medium …

Background Retarded embryo growth is a pervasive effect of culture in vitro. Methods A
systematic analysis of the interactions between media design, embryo culture density …

The culture of preimplantation embryos in vitro is an important method for human and mouse
reproductive technology. This study aims to investigate the influence of different conditions …

We report here on an improved, completely defined culture system for producing embryos in
vitro which mimics development in vivo. This system avoids the confounding effects of the …

Further refinement of culture media is needed to improve the quality of embryos generated
in vitro. Previous results from our laboratory demonstrated that uptake of nutrients by the …