Yeasts represent a group of the microorganisms most frequently seen in biotechnology.
Recently, the class 2 type II CRISPR system (CRISPR/Cas9) has become the principal …

The recent discovery and use of CRISPR/Cas9 gene editing technology has provided new
opportunities for scientific research in many fields of study including agriculture, genetic …

Saccharomyces cerevisiae has been for a long time a common model for fundamental
biological studies and a popular biotechnological engineering platform to produce …

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome
engineering, while also changing the speed and efficiency of metabolic engineering in …

CRISPR-Cas has become a powerful technique for genetic engineering of yeast. Here, we
present an improved version by using only one single plasmid expressing Cas9 and one or …

Non-conventional yeasts have attracted a growing interest on account of their excellent
characteristics. In recent years, the emerging of CRISPR/Cas technology has improved the …

Recent advances in CRISPR/Cas9 based genome editing have considerably advanced
genetic engineering of industrial yeast strains. In this study, we report the construction and …

The CRISPR/Cas9 systems have been developed as tools for genetic engineering and
metabolic engineering in various organisms. In this review, various aspects of CRISPR/Cas9 …

Synthetic yeast cell factories provide a remarkable solution for the sustainable supply of a
range of products, ranging from large-scale industrial chemicals to high-value …

Genome editing using the CRISPR/Cas system has been implemented for various
organisms and becomes increasingly popular even in the genetically tractable budding …