Expanding the scope of CRISPR/Cpf1-mediated genome editing in rice
The CRISPR/Cas gene editing system offers great potential for functional genomics in plants
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
and crop improvement. The specificity of Cas-directed DNA cleavage is strictly determined …
Expanding the range of CRISPR/Cas9 genome editing in rice
The CRISPR/Cas9 system has emerged as a versatile molecular tool for genome editing in
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
various organisms, including plants. In this system, the specificity of Cas9-directed DNA …
[HTML][HTML] Enhanced genome editing in rice using single transcript unit CRISPR‐LbCpf1 systems
A variety of sequence-specific nucleases (SSNs) have been successfully used for plant
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …
genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR) …
[HTML][HTML] xCas9 expands the scope of genome editing with reduced efficiency in rice
J Wang, X Meng, X Hu, T Sun, J Li… - Plant biotechnology …, 2019 - ncbi.nlm.nih.gov
CRISPR-Cas9 systems have been widely used in animals and plants for genome editing,
epigenetic modification, etc.(Cong et al., 2013; Hilton et al., 2015). However, the accurate …
epigenetic modification, etc.(Cong et al., 2013; Hilton et al., 2015). However, the accurate …
Multiplex gene editing in rice using the CRISPR-Cpf1 system
M Wang, Y Mao, Y Lu, X Tao, J Zhu - Molecular plant, 2017 - cell.com
The class 2/type II clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9
system has been used successfully for simultaneous modification of multiple loci in plants …
system has been used successfully for simultaneous modification of multiple loci in plants …
Generation of targeted point mutations in rice by a modified CRISPR/Cas9 system
CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-
associated Cas9 endonuclease)-mediated genome editing has revolutionized biological …
associated Cas9 endonuclease)-mediated genome editing has revolutionized biological …
[PDF][PDF] Robust genome editing of CRISPR-Cas9 at NAG PAMs in rice
The CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats/Cas9) system
has been widely used for a variety of applications, including targeted gene knockout, gene …
has been widely used for a variety of applications, including targeted gene knockout, gene …
[HTML][HTML] Targeted base editing in rice with CRISPR/ScCas9 system
M Wang, Z Xu, G Gosavi, B Ren, Y Cao… - Plant Biotechnology …, 2020 - ncbi.nlm.nih.gov
The CRISPR/Cas system has rapidly become the preferred tool for genome engineering in
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …
various organisms due to high efficiency, specificity, simplicity and versatility. Currently …
Genome engineering in rice using Cas9 variants that recognize NG PAM sequences
K Hua, X Tao, P Han, R Wang, JK Zhu - Molecular plant, 2019 - cell.com
CRISPR/Cas9 genome editing relies on sgRNA-target DNA base pairing and a short
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …
downstream PAM sequence to recognize target DNA. The strict protospacer adjacent motif …
CRISPR-GE: a convenient software toolkit for CRISPR-based genome editing
Use of the clustered regularly interspaced short palindromic repeat (CRISPR)-associated
protein9 (Cas9) and Cpf1 systems in plants (Ma et al., 2016; Wang et al., 2017) involves …
protein9 (Cas9) and Cpf1 systems in plants (Ma et al., 2016; Wang et al., 2017) involves …