In vitro characterization of tumor cell biology or of potential anticancer drugs is usually
performed using tumor cell lines cultured as a monolayer. However, it has been previously …

Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid
tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent …

To obtain a multicellular MCF-7 spheroid model to mimic the three-dimensional (3D) of
tumors, the microwell liquid overlay (A) and hanging-drop/agar (B) methods were first …

Owing to their in vivo-like characteristics, three-dimensional (3 D) multicellular tumor
spheroid (MCTS) cultures are gaining increasing popularity as an in vitro model of tumors. A …

Spheroid culture is a widely used three-dimensional culture technology that simulates the
three-dimensional structure of tumors in vivo and has been considered a good model for …

ABSTRACT Three‐dimensional (3D) cell culture models of solid tumors are currently having
a tremendous impact in the in vitro screening of candidate anti‐tumoral therapies. These 3D …

The preclinical drug screening of pancreatic cancer treatments suffers from the absence of
appropriate models capable to reproduce in vitro the heterogeneous tumor …

Cellular spheroids serving as three-dimensional (3D) in vitro tissue models have attracted
increasing interest for pathological study and drug-screening applications. Various methods …

There is increasing recognition that three-dimensional (3D) tissue culture technologies have
many uses within the biomedical sciences beyond the scope of regenerative medicine. One …

The multicellular tumor spheroid model is a widely used culture system to mimic the three-
dimensionality of tumors. Several methods and an even larger number of protocols are …