The lagging annotation of bacterial genomes and the inherent genetic complexity of many
phenotypes is hindering the discovery of new drug targets and the development of new …

Without a doubt, in the past 20 or so years, we have achieved the power of biology in
different ways. In the present, we have many tools for developing novel technologies and …

Genome engineering of non-conventional microorganisms calls for the development of
dedicated synthetic biology tools. Pseudomonas putida is a Gram-negative, non-pathogenic …

Abstract BLAST (Basic Local Alignment Search Tool) searches against DNA and protein
sequence databases have become an indispensable tool for biomedical research. The …

VRprofile is a Web server that facilitates rapid investigation of virulence and antibiotic
resistance genes, as well as extends these trait transfer-related genetic contexts, in newly …

Genotyping methods and genome sequencing are indispensable to reveal genomic
structure of bacterial species displaying high level of genome plasticity. However …

Plasmids are used extensively in basic and applied biology. However, design and
construction of plasmids, specifically the ones carrying complex genetic information, remains …

The demand for plasmid DNA (pDNA) is anticipated to increase significantly as DNA
vaccines and non-viral gene therapies enter phase 3 clinical trials and are approved for use …

Background Evolvability is an intrinsic feature of all living cells. However, newly emerging,
evolved features can be undesirable when genetic circuits, designed and fabricated by …

Abstract The Alliance of Genome Resources (Alliance) is a consortium of the major model
organism databases and the Gene Ontology that is guided by the vision of facilitating …