Owing to their in vivo-like characteristics, three-dimensional (3 D) multicellular tumor
spheroid (MCTS) cultures are gaining increasing popularity as an in vitro model of tumors. A …

Multicellular tumor spheroids (MCTS) are used as organotypic models of normal and solid
tumor tissue. Traditional techniques for generating MCTS, such as growth on nonadherent …

The establishment of a vascular network within tumours is a key step in the progression
towards an aggressive, metastatic state, with poor prognosis. We have developed a novel in …

Many types of mammalian cells can aggregate and differentiate into 3‐D multicellular
spheroids when cultured in suspension or a nonadhesive environment. Compared to …

Recently, many types of in vitro 3‐D culture systems have been developed to recapitulate
the in vivo growth conditions of cancer. The cancer 3‐D culture methods aim to preserve the …

The growth of tumour cells as three‐dimensional multicellular spheroids in vitro has led to
important insights in tumour biology, since properties of the in vivo‐tumour such as …

In vitro characterization of tumor cell biology or of potential anticancer drugs is usually
performed using tumor cell lines cultured as a monolayer. However, it has been previously …

Techniques that allow cells to self-assemble into three-dimensional (3-D) spheroid
microtissues provide powerful in vitro models that are becoming increasingly popular …

During the last three decades multicellular spheroids of both normal and malignant cells
have been used as model systems for studying three-dimen sional growth and differentiation …

Abstract Background aims. The 3-dimensional (3-D) culture of various cell types reflects the
in vivo situation more precisely than 2-dimensional (2-D) cell culture techniques. Spheroids …