It has been reported that buffalo (Bubalus bubalis) embryos reconstructed by somatic cell
nucleus transfer (SCNT) can develop to the full term of gestation and result in newborn …

Conteúdo: Acetylation of histones is a major mechanism of genome epigenetic
reprogramming of the gametes in order to establish a totipotent state to the normal …

Objective: To evaluate the effect of treatment with trichostatin-A (TSA) on the production of
bovine embryos, expressing the gene of the green fluorescent protein (GFP) generated by …

Cloning mammals by somatic cell nuclear transfer (SCNT) has become an established
procedure, but the success rate remains low and gene expression abnormalities are also …

Developmental potential of cloned embryos is related closely to epigenetic modification of
somatic cell genome. The present study was to investigate the effects of applying histone …

Somatic cell nuclear transfer (SCNT) provides a unique opportunity to directly produce a
cloned animal from a donor cell, and it requires the use of skillful techniques. Additionally …

Previous studies in the mouse have shown treatment of somatic cell nuclear transfer (SCNT)
embryos with histone deacetylase inhibitors (HDACi) to significantly increase cloning …

Abnormal epigenetic modification is supposed to be one of factors accounting for inefficient
reprogramming of the donor cell nuclei in ooplasm after somatic cell nuclear transfer …

Faulty epigenetic reprogramming of somatic nuclei is likely to be a major cause of low
success observed in all mammals produced through somatic cell nuclear transfer (SCNT). It …

Fibroblasts and cumulus cells are more prone to be reprogrammed than other cell types,
and the reprogramming ability of donor cells will be improved after treated with Trichostatin …