Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants

P Umbeck, G Johnson, K Barton, W Swain - Bio/technology, 1987 - nature.com
Genetically engineered plants of commercial cotton varieties were obtained by
Agrobacterium–mediated transformation. Inoculated tissues selected on kanamycin …

Transformation of cotton (Gossypium hirsutum L.) by Agrobacterium tumefaciens and regeneration of transgenic plants

E Firoozabady, DL DeBoer, DJ Merlo, EL Halk… - Plant molecular …, 1987 - Springer
Cotton (Gossypium hirsutum L.) cotyledon tissues have been efficiently transformed and
plants have been regenerated. Cotyledon pieces from 12-day-old aseptically germinated …

Tissue culture-independent in planta transformation strategy: an Agrobacterium tumefaciens-mediated gene transfer method to overcome recalcitrance in cotton …

E Keshamma, S Rohini, K Sankara Rao… - Journal of cotton …, 2008 - repository.ias.ac.in
The major constraint in cotton improvement has been the recalcitrance of cotton varieties to
tissue culture. Alternate methods that avoid/minimize tissue culture would be beneficial for …

A simple and rapid Agrobacterium-mediated transformation protocol for cotton (Gossypium hirsutum L.): Embryogenic calli as a source to generate large numbers of …

S Leelavathi, VG Sunnichan, R Kumria, GP Vijaykanth… - Plant cell reports, 2004 - Springer
A protocol is presented for efficient transformation and regeneration of cotton. Embryogenic
calli co-cultivated with Agrobacterium carrying cry1Ia5 gene were cultured under …

[PDF][PDF] Adaptation of cotton shoot apex culture to Agrobacterium-mediated transformation

JH Gould, M Magallanes-Cedeno - Plant molecular biology reporter, 1998 - Citeseer
A protocol is presented for rapid genotype-independent transformation and regeneration of
cotton (Gossypium spp.) from shoots isolated from germinating seedlings. Isolated shoots …

High efficiency transformation protocol for three Indian cotton varieties via Agrobacterium tumefaciens

VV Satyavathi, V Prasad, BG Lakshmi, GL Sita - Plant science, 2002 - Elsevier
A protocol for consistent production of transgenic cotton plants in three Indian varieties was
established utilizing Agrobacterium-mediated transformation. Shoot tip explants were …

Transgenic cotton: factors influencing Agrobacterium-mediated transformation and regeneration

G Sunilkumar, KS Rathore - Molecular Breeding, 2001 - Springer
Various aspects of transformation and regeneration processes were examined in efforts to
improve the efficiency of production of transgenic cotton (Gossypium hirsutum L.). Green …

Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex

C Zapata, SH Park, KM El-Zik, RH Smith - Theoretical and Applied …, 1999 - Springer
Transgenic cotton (Gossypium hirsutum L.) plants of a Texas cultivar CUBQHRPIS were
obtained using Agrobacterium-mediated transformation coupled with the use of shoot-apex …

Factors affecting transformation efficiency of embryogenic callus of Upland cotton (Gossypium hirsutum) with Agrobacterium tumefaciens

S Jin, X Zhang, S Liang, Y Nie, X Guo… - Plant cell, tissue and …, 2005 - Springer
A reliable and high-efficiency system of transforming embryogenic callus (EC) mediated by
Agrobacterium tumefaciens was developed in cotton. Various aspects of transformation …

Agrobacterium -mediated transformation of cotton (Gossypium hirsutum) using a heterologous bean chitinase gene

M Tohidfar, M Mohammadi, B Ghareyazie - Plant cell, tissue and organ …, 2005 - Springer
Cotton (Gossypium hirsutum L., var. Coker 312) hypocotyl explants were transformed with
three strains of Agrobacterium tumefaciens, LBA4404, EHA101 and C58, each harboring the …