The serum-free culture of multicellular tumor spheroids from two rat (BT4c, BT5c) and two
human (GaMg, D-54Mg) glioma cell lines is described. The spheroids were propagated in …

Pioneered by the work of two developmental biologists, J. Holtfreter and A. Moscona, in the
1940s and 1950s, the methodology of cell aggregates was adapted to cancer research by …

The tumor in vivo has often a nodular or spheroidal appearance, as shown by Thomlinson
and Gray (1955) and Tannock (1968), with viable cells surrounding penetrating capillaries …

THIS COMMENTARY is focussed on recent publications about tumour spheroid technology
in cancer therapy research and mainly on those publications describing the effects of …

EMT6 mammary sarcoma cells were grown in vitro as multicellular spheroids to model for
the heterogeneity of microenvironments and structural changes which develop in many …

This chapter provides a brief survey of the methods for culturing multicellular tumor
spheroids and explains how tumor spheroids can be used in various experimental studies. It …

Recent research using multicellular tumor spheroids has resulted in new insights in the
regulation of invasion and metastasis, angiogenesis and cell cycle kinetics. The onset and …

Cancer is a complex disease in which both genetic defects and microenvironmental
components contribute to the development, progression, and metastasization of disease …

Multicellular tumour spheroids were prepared from a total of 46 human brain tumour
biopsies by collagenase digestion and plating into agar coated flasks. Both primary …

Multicell spheroids provide an in vitro tumour model of intermediate complexity between
tumours and standard cell cultures. Spheroids of several varieties of normal and tumour …