Effects of knocking out three anthocyanin modification genes on the blue pigmentation of gentian flowers

K Tasaki, A Higuchi, A Watanabe, N Sasaki… - Scientific Reports, 2019 - nature.com
Genome editing by the CRISPR/Cas9 system has recently been used to produce gene
knockout lines in many plant species. We applied this system to analyze Japanese gentian …

Genetic engineering of novel flower colour by suppression of anthocyanin modification genes in gentian

T Nakatsuka, K Mishiba, A Kubota, Y Abe… - Journal of plant …, 2010 - Elsevier
Ornamental gentian plants have vivid-blue flowers. The main factor contributing to the flower
colour is the accumulation of a polyacylated delphinidin 'gentiodelphin'in their petals …

Molecular characterization of an anthocyanin-related glutathione S-transferase gene in Japanese gentian with the CRISPR/Cas9 system

K Tasaki, M Yoshida, M Nakajima, A Higuchi… - BMC Plant …, 2020 - Springer
Background The blue pigmentation of Japanese gentian flowers is due to a polyacylated
anthocyanin, gentiodelphin, and all associated biosynthesis genes and several regulatory …

Anthocyanin biosynthesis genes as model genes for genome editing in plants

E Khusnutdinov, A Sukhareva, M Panfilova… - International journal of …, 2021 - mdpi.com
CRISPR/Cas, one of the most rapidly developing technologies in the world, has been
applied successfully in plant science. To test new nucleases, gRNA expression systems and …

Application of the CRISPR/Cas9 system for modification of flower color in Torenia fournieri

M Nishihara, A Higuchi, A Watanabe, K Tasaki - BMC Plant Biology, 2018 - Springer
Background CRISPR/Cas9 technology is one of the most powerful and useful tools for
genome editing in various living organisms. In higher plants, the system has been widely …

Generation of blue chrysanthemums by anthocyanin B-ring hydroxylation and glucosylation and its coloration mechanism

N Noda, S Yoshioka, S Kishimoto, M Nakayama… - Science …, 2017 - science.org
Various colored cultivars of ornamental flowers have been bred by hybridization and
mutation breeding; however, the generation of blue flowers for major cut flower plants, such …

First genome edited poinsettias: targeted mutagenesis of flavonoid 3′-hydroxylase using CRISPR/Cas9 results in a colour shift

D Nitarska, R Boehm, T Debener, RC Lucaciu… - Plant Cell, Tissue and …, 2021 - Springer
The CRISPR/Cas9 system is a remarkably promising tool for targeted gene mutagenesis,
and becoming ever more popular for modification of ornamental plants. In this study we …

CRISPR/Cas9-mediated mutagenesis of the dihydroflavonol-4-reductase-B (DFR-B) locus in the Japanese morning glory Ipomoea (Pharbitis) nil

K Watanabe, A Kobayashi, M Endo, K Sage-Ono… - Scientific reports, 2017 - nature.com
CRISPR/Cas9 technology is a versatile tool for targeted mutagenesis in many organisms,
including plants. However, this technique has not been applied to the Japanese morning …

A rationale for the shift in colour towards blue in transgenic carnation flowers expressing the flavonoid 3′, 5′-hydroxylase gene

Y Fukui, Y Tanaka, T Kusumi, T Iwashita, K Nomoto - Phytochemistry, 2003 - Elsevier
Recently marketed genetically modified violet carnations cv. Moondust and Moonshadow
(Dianthus caryophyllus) produce a delphinidin type anthocyanin that native carnations …

Repurposing of anthocyanin biosynthesis for plant transformation and genome editing

Y He, M Zhu, J Wu, L Ouyang, R Wang… - Frontiers in genome …, 2020 - frontiersin.org
CRISPR/Cas9 gene editing technology has been very effective in editing genes in many
plant species including rice. Here we further improve the current CRISPR/Cas9 gene editing …