Membrane protein structural biology is a frontier area of modern biomedical research.
Twenty to thirty-five percent of the proteins encoded by an organism's genome are integral …

Integral membrane proteins present unparalleled challenges for structural genomics
programs. Samples from this class of proteins are not only difficult to produce in quantities …

Cell-free expression techniques have emerged as promising tools for the production of
membrane proteins for structural and functional analysis. Elimination of toxic effects and a …

Membrane proteins (MPs) are essential components of all biological membranes,
contributing to key cellular functions that include signalling, molecular transport and energy …

Crystallisation of integral membranes requires milligrams of purified protein in a
homogeneous, monodisperse state, and crucially, the membrane protein must also be fully …

Historically, recombinant membrane protein production has been a major challenge
meaning that many fewer membrane protein structures have been published than those of …

Highlights•Membrane–proteins exist in complexes.•The appropriate expression host is key
to produce membrane–proteins.•Designed bacterial strains and baculovirus-insect cell …

Atomic-resolution structural studies of membrane-associated proteins and peptides in a
membrane environment are important to fully understand their biological function and the …

α-Helical membrane proteins are important for many biological functions. Due to
physicochemical constraints, the structures of membrane proteins differ from the structure of …

Improvements in the fields of membrane-protein molecular biology and biochemistry,
technical advances in structural data collection and processing, and the availability of …