Microfluidic Chips for In Vivo Imaging of Cellular Responses to Neural Injury in Drosophila Larvae

M Ghannad-Rezaie, X Wang, B Mishra, C Collins… - PloS one, 2012 - journals.plos.org
With powerful genetics and a translucent cuticle, the Drosophila larva is an ideal model
system for live imaging studies of neuronal cell biology and function. Here, we present an …

Using microfluidics chips for live imaging and study of injury responses in Drosophila larvae

B Mishra, M Ghannad-Rezaie, J Li, X Wang… - JoVE (Journal of …, 2014 - jove.com
Live imaging is an important technique for studying cell biological processes, however this
can be challenging in live animals. The translucent cuticle of the Drosophila larva makes it …

[HTML][HTML] A Drosophila in vivo injury model for studying neuroregeneration in the peripheral and central nervous system

D Li, F Li, P Guttipatti, Y Song - Journal of Visualized Experiments …, 2018 - ncbi.nlm.nih.gov
The regrowth capacity of damaged neurons governs neuroregeneration and functional
recovery after nervous system trauma. Over the past few decades, various intrinsic and …

Design and implementation of in vivo imaging of neural injury responses in the adult Drosophila wing

Y Fang, L Soares, NM Bonini - Nature protocols, 2013 - nature.com
Live-imaging technology has markedly advanced in the field of neural injury and axon
degeneration; however, studies are still predominantly performed in in vitro settings such as …

An Immobilization Technique for Long-Term Time-Lapse Imaging of Explanted Drosophila Tissues

MP Bostock, AR Prasad, R Chaouni… - Frontiers in Cell and …, 2020 - frontiersin.org
Time-lapse imaging is an essential tool to study dynamic biological processes that cannot
be discerned from fixed samples alone. However, imaging cell-and tissue-level processes in …

Long-term in vivo imaging of Drosophila larvae

P Kakanj, SA Eming, L Partridge, M Leptin - Nature Protocols, 2020 - nature.com
The Drosophila larva has been used to investigate many processes in cell biology, including
morphogenesis, physiology and responses to drugs and new therapeutic compounds …

[HTML][HTML] Whole cell recordings from brain of adult Drosophila

H Gu, DK O'Dowd - Journal of Visualized Experiments: JoVE, 2007 - ncbi.nlm.nih.gov
In this video, we demonstrate the procedure for isolating whole brains from adult Drosophila
in preparation for recording from single neurons. We begin by describing the dissecting …

Harvesting and Preparing Drosophila Embryos for Electrophysiological Recording and Other Procedures

DE Featherstone, K Chen, K Broadie - JoVE (Journal of Visualized …, 2009 - jove.com
Drosophila is a premier genetic model for the study of both embryonic development and
functional neuroscience. Traditionally, these fields are quite isolated from each other, with …

Live imaging of Drosophila imaginal disc development

S Aldaz, LM Escudero… - Proceedings of the …, 2010 - National Acad Sciences
Live imaging has revolutionized the analysis of developmental biology over the last few
years. The ability to track in real time the dynamic processes that occur at tissue and cellular …

Preparation of developing and adult Drosophila brains and retinae for live imaging

WR Williamson, PR Hiesinger - JoVE (Journal of Visualized Experiments), 2010 - jove.com
The Drosophila brain and visual system are widely utilized model systems to study neuronal
development, function and degeneration. Here we show three preparations of the brain and …