GO: a functional reporter system to identify and enrich base editing activity

A Katti, M Foronda, J Zimmerman, B Diaz… - Nucleic acids …, 2020 - academic.oup.com
Base editing (BE) is a powerful tool for engineering single nucleotide variants (SNVs) and
has been used to create targeted mutations in cell lines, organoids and animal models …

[HTML][HTML] Methods and applications of CRISPR-mediated base editing in eukaryotic genomes

GT Hess, J Tycko, D Yao, MC Bassik - Molecular cell, 2017 - cell.com
The past several years have seen an explosion in development of applications for the
CRISPR-Cas9 system, from efficient genome editing, to high-throughput screening, to …

Small-molecule inhibitors of histone deacetylase improve CRISPR-based adenine base editing

HR Shin, JE See, J Kweon, HS Kim… - Nucleic Acids …, 2021 - academic.oup.com
CRISPR-based base editors (BEs) are widely used to induce nucleotide substitutions in
living cells and organisms without causing the damaging DNA double-strand breaks and …

Development of a simple and quick method to assess base editing in human cells

X Lv, K Qiu, T Tu, X He, Y Peng, J Ye, J Fu… - … Therapy-Nucleic Acids, 2020 - cell.com
Base editing is a form of genome editing that can directly convert a single base (C or A) to
another base (T or G), which is of great potential in biomedical applications. The broad …

In Vivo Rapid Investigation of CRISPR-Based Base Editing Components in Escherichia coli (IRI-CCE): A Platform for Evaluating Base Editing Tools and Their …

RM Shelake, D Pramanik, JY Kim - International Journal of Molecular …, 2022 - mdpi.com
Rapid assessment of clustered regularly interspaced short palindromic repeats/CRISPR-
associated protein (CRISPR/Cas)-based genome editing (GE) tools and their components is …

Pioneer factor improves CRISPR‐based C‐To‐G and C‐To‐T base editing

C Yang, X Dong, Z Ma, B Li, C Bi, X Zhang - Advanced Science, 2022 - Wiley Online Library
Base editing events in eukaryote require a compatible chromatin environment, but there is
little research on how chromatin factors contribute to the editing efficiency or window. By …

Web-based design and analysis tools for CRISPR base editing

GH Hwang, J Park, K Lim, S Kim, J Yu, E Yu, ST Kim… - BMC …, 2018 - Springer
Background As a result of its simplicity and high efficiency, the CRISPR-Cas system has
been widely used as a genome editing tool. Recently, CRISPR base editors, which consist …

CRISPR DNA base editors with reduced RNA off-target and self-editing activities

J Grünewald, R Zhou, S Iyer, CA Lareau… - Nature …, 2019 - nature.com
Cytosine or adenine base editors (CBEs or ABEs) can introduce specific DNA C-to-T or A-to-
G alterations,,–. However, we recently demonstrated that they can also induce transcriptome …

Innate programmable DNA binding by CRISPR-Cas12m effectors enable efficient base editing

G Bigelyte, B Duchovska, R Zedaveinyte… - Nucleic Acids …, 2024 - academic.oup.com
Cas9 and Cas12 nucleases of class 2 CRISPR-Cas systems provide immunity in
prokaryotes through RNA-guided cleavage of foreign DNA. Here we characterize a set of …

Effective gene editing by high-fidelity base editor 2 in mouse zygotes

P Liang, H Sun, Y Sun, X Zhang, X Xie, J Zhang… - Protein & …, 2017 - academic.oup.com
Targeted point mutagenesis through homologous recombination has been widely used in
genetic studies and holds considerable promise for repairing disease-causing mutations in …