Publisher Summary Dictyostelium discoideum is a haploid organism. This allows direct
isolation of mutants. A parasexual genetic system has been established and has been …

Monoclonal antibodies against actin-binding proteins from the slime mould Dictyostelium
discoideum were used to screen mutagenized cells in an attempt to select strains defective …

In many nonmuscle cells, actin filaments are located underneath the plasma membrane in a
region called the cortex. During cell locomotion a reorganization of the cortex is expected to …

The contribution of three actin cross-linking proteins, α-actinin (αA), gelation factor (ABP-
120), and the 34 kDa actin-bundling protein to cellular functions has been studied in three …

The cellular slime mold Dictyostelium discoideum is becoming the premier system for the
explication of the biochemical and cellular events that occur during motile processes …

A clone coding for the F-actin cross-linking protein alpha-actinin was obtained by screening
a genomic library of Dictyostelium discoideum DNA in lambda gt11 with monoclonal …

The actin cytoskeleton is implicated in many cellular processes, such as cell adhesion,
locomotion, contraction and cytokinesis, which are central to any development. The extent of …

We generated by gene disruption Dictyostelium cells that lacked both the F-actin cross-
linking proteins, a-actinin and gelation factor. Several major cell functions, such as growth …

A monoclonal antibody, mAb 47‐19‐2, was used to study the subunit topology of the rod‐
shaped alpha‐actinin molecules of Dictyostelium discoideum and to screen for mutants …

Actin-binding proteins are known to regulate in vitro the assembly of actin into
supramolecular structures, but evidence for their activities in living nonmuscle cells is …