[HTML][HTML] Cytokine RT-qPCR and ddPCR for immunological investigations of the endangered Australian sea lion (Neophoca cinerea) and other mammals

MIM Cerda, R Gray, DP Higgins - PeerJ, 2020 - peerj.com
Measurement of cytokine gene expression by reverse transcription quantitative polymerase
chain reaction (RT-qPCR) is used widely to assess the immune system of animals and to …

[HTML][HTML] Developing Immune Profiles of Endangered Australian Sea Lion (Neophoca cinerea) Pups Within the Context of Endemic Hookworm (Uncinaria sanguinis) …

MI Meza Cerda, R Gray, PC Thomson… - Frontiers in Veterinary …, 2022 - frontiersin.org
As a top predator, the endangered Australian sea lion (Neophoca cinerea) is a sentinel of
ecosystem change, where population trends can reflect broader shifts in the marine …

[HTML][HTML] Monitoring bottlenose dolphin leukocyte cytokine mRNA responsiveness by qPCR

AR Hofstetter, KC Eberle, SK Venn-Watson… - PloS one, 2017 - journals.plos.org
Both veterinarians caring for dolphins in managed populations and researchers monitoring
wild populations use blood-based diagnostics to monitor bottlenose dolphin (Tursiops …

[HTML][HTML] Expression profiles of the immune genes CD4, CD8β, IFNγ, IL-4, IL-6 and IL-10 in mitogen-stimulated koala lymphocytes (Phascolarctos cinereus) by qRT …

IE Maher, JE Griffith, Q Lau, T Reeves, DP Higgins - PeerJ, 2014 - peerj.com
Investigation of the immune response of the koala (Phascolarctos cinereus) is needed
urgently, but has been limited by scarcity of species-specific reagents and methods for this …

[HTML][HTML] Development and testing of species-specific ELISA assays to measure IFN-γ and TNF-α in bottlenose dolphins (Tursiops truncatus)

KC Eberle, SK Venn-Watson, ED Jensen, J LaBresh… - Plos one, 2018 - journals.plos.org
Monitoring the immune status of cetaceans is important for a variety of health conditions.
Assays to quantify cytokines, especially pro-inflammatory cytokines, could be employed, in …

Development of reverse-transcriptase quantitative PCR assays for detection of the cytokines IL-1β, TNF-α, and IL-10 in chelonians

JM Rayl, JFX Wellehan Jr, D Bunick, MC Allender - Cytokine, 2019 - Elsevier
In response to viral pathogens, a host releases pro-inflammatory cytokines such as
interleukin-1 beta (IL-1β) and tumor necrosis factor alpha (TNF-α) and anti-inflammatory …

[PDF][PDF] Recent Cytokine Findings and Implications Towards Health Assessment of the Bottlenose Dolphin (Tursiops truncatus)

A Vecchione, MM Peden-Adams, TA Romano… - Aquatic …, 2008 - academia.edu
Bottlenose dolphins (Tursiops truncatus) are toplevel marine predators and represent a
sentinel species for ocean health. The study of cytokines and immunocellular function are …

[HTML][HTML] A probe-based qRT-PCR method to profile immunological gene expression in blood of captive beluga whales (Delphinapterus leucas)

MA Tsai, IH Chen, JH Wang, SJ Chou, TH Li, MY Leu… - PeerJ, 2017 - peerj.com
Cytokines are fundamental for a functioning immune system, and thus potentially serve as
important indicators of animal health. Quantitation of mRNA using quantitative reverse …

Development and evaluation of a SYBR Green real-time RT-PCR assay for evaluation of cytokine gene expression in horse

A Sánchez-Matamoros, D Kukielka, AI De las Heras… - Cytokine, 2013 - Elsevier
Cytokine secretion is one of the main mechanisms by which the immune system is regulated
in response to pathogens. Therefore, the measurement of cytokine expression is …

Validation of a commercial canine assay kit to measure pinniped cytokines

M Levin, T Romano, K Matassa, S De Guise - Veterinary Immunology and …, 2014 - Elsevier
The present study was conducted to assess and validate the cross-reactivity of commercially
available multiplex human and canine cytokine kits coupled with the Bio-Plex 200 platform …