CRISPR-RT: a web application for designing CRISPR-C2c2 crRNA with improved target specificity
CRISPR-Cas systems have been successfully applied in genome editing. Recently, the
CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe …
CRISPR-C2c2 system has been reported as a tool for RNA editing. Here we describe …
CRISPR guide RNA design guidelines for efficient genome editing
The simple applicability and facile target programming of the CRISPR/Cas9-system abolish
the major boundaries of previous genome editing tools, making it the tool of choice for …
the major boundaries of previous genome editing tools, making it the tool of choice for …
Overview of guide RNA design tools for CRISPR-Cas9 genome editing technology
LJ Zhu - Frontiers in Biology, 2015 - Springer
Abstract CRISPR-Cas (Clustered, Regularly Interspaced, Short Palindromic Repeats–
CRISPR-associated (Cas)) RNA guided endonuclease has emerged as the most effective …
CRISPR-associated (Cas)) RNA guided endonuclease has emerged as the most effective …
Harnessing noncanonical crRNA for highly efficient genome editing
The CRISPR-Cas12a system is more advantageous than the widely used CRISPR-Cas9
system in terms of specificity and multiplexibility. However, its on-target editing efficiency is …
system in terms of specificity and multiplexibility. However, its on-target editing efficiency is …
Web-based CRISPR toolkits: Cas-OFFinder, cas-designer, and cas-analyzer
The CRISPR-Cas system facilitates highly efficient genome editing; thus, it has been applied
in many research fields such as biological science, medicine, and gene therapy. However …
in many research fields such as biological science, medicine, and gene therapy. However …
CRISPR–Cas9-mediated genome editing and guide RNA design
CRISPR and CRISPR-associated (Cas) proteins, which in nature comprise the RNA-based
adaptive immune system in bacteria and archaea, have emerged as particularly powerful …
adaptive immune system in bacteria and archaea, have emerged as particularly powerful …
CHOPCHOP v2: a web tool for the next generation of CRISPR genome engineering
In just 3 years CRISPR genome editing has transformed biology, and its popularity and
potency continue to grow. New CRISPR effectors and rules for locating optimum targets …
potency continue to grow. New CRISPR effectors and rules for locating optimum targets …
CaSilico: A versatile CRISPR package for in silico CRISPR RNA designing for Cas12, Cas13, and Cas14
A Asadbeigi, M Norouzi, MS Vafaei Sadi… - … in Bioengineering and …, 2022 - frontiersin.org
The efficiency of the CRISPR-Cas system is highly dependent on well-designed CRISPR
RNA (crRNA). To facilitate the use of various types of CRISPR-Cas systems, there is a need …
RNA (crRNA). To facilitate the use of various types of CRISPR-Cas systems, there is a need …
CASowary: CRISPR-Cas13 guide RNA predictor for transcript depletion
Background Recent discovery of the gene editing system-CRISPR (Clustered Regularly
Interspersed Short Palindromic Repeats) associated proteins (Cas), has resulted in its …
Interspersed Short Palindromic Repeats) associated proteins (Cas), has resulted in its …
Highly efficient genome editing by CRISPR-Cpf1 using CRISPR RNA with a uridinylate-rich 3′-overhang
SU Bin Moon, JM Lee, JG Kang, NE Lee, DI Ha… - Nature …, 2018 - nature.com
Genome editing has been harnessed through the development of CRISPR system, and the
CRISPR from Prevotella and Francisella 1 (Cpf1) system has emerged as a promising …
CRISPR from Prevotella and Francisella 1 (Cpf1) system has emerged as a promising …