The CRISPR–Cas system for plant genome editing: advances and opportunities

V Kumar, M Jain - Journal of experimental botany, 2015 - academic.oup.com
Genome editing is an approach in which a specific target DNA sequence of the genome is
altered by adding, removing, or replacing DNA bases. Artificially engineered hybrid …

Targeted gene manipulation in plants using the CRISPR/Cas technology

D Zhang, Z Li, JF Li - Journal of Genetics and Genomics, 2016 - Elsevier
The CRISPR/Cas technology is emerging as a revolutionary genome editing tool in diverse
organisms including plants, and has quickly evolved into a suite of versatile tools for …

[HTML][HTML] CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation

T Cardi, J Murovec, A Bakhsh, J Boniecka… - Trends in Plant …, 2023 - cell.com
The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

CRISPR/Cas9-mediated genome editing and gene replacement in plants: transitioning from lab to field

SM Schaeffer, PA Nakata - Plant Science, 2015 - Elsevier
The CRISPR/Cas9 genome engineering system has ignited and swept through the scientific
community like wildfire. Owing largely to its efficiency, specificity, and flexibility, the …

[HTML][HTML] The CRISPR/Cas9 system for plant genome editing and beyond

L Bortesi, R Fischer - Biotechnology advances, 2015 - Elsevier
Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

CRISPR/Cas9: an advanced tool for editing plant genomes

MK Samanta, A Dey, S Gayen - Transgenic research, 2016 - Springer
To meet current challenges in agriculture, genome editing using sequence-specific
nucleases (SSNs) is a powerful tool for basic and applied plant biology research. Here, we …

[HTML][HTML] Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

K Belhaj, A Chaparro-Garcia, S Kamoun, V Nekrasov - Plant methods, 2013 - Springer
Targeted genome engineering (also known as genome editing) has emerged as an
alternative to classical plant breeding and transgenic (GMO) methods to improve crop …

Recent advances in CRISPR/Cas mediated genome editing for crop improvement

S Sharma, R Kaur, A Singh - Plant biotechnology reports, 2017 - Springer
Plant breeding is one of the oldest sustainable agriculture methods used to increase the
yield, quality and other biomaterial for human use. Many crops like fruits, vegetables …

[HTML][HTML] Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering

N Wada, R Ueta, Y Osakabe, K Osakabe - BMC Plant Biology, 2020 - Springer
Traditionally, generation of new plants with improved or desirable features has relied on
laborious and time-consuming breeding techniques. Genome-editing technologies have led …

[HTML][HTML] CRISPR-Cas9 system for plant genome editing: Current approaches and emerging developments

JAV Montecillo, LL Chu, H Bae - Agronomy, 2020 - mdpi.com
Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic
engineering tool in various biological systems. This editing technology has been in the …